استخراج گزينشي و اندازه گيري داروي ساليسيليك اسيد در پلاسما و ادرار انسان به كمك روش پليمر حكاكي شده مولكولي - طيف سنج تحرك يوني با منبع يونيزاسيون الكترواسپري

چكيده انگليسي :

Determination of Salicylic Acid Drug in Plasma and Urine Samples using Technique of Molcularly Imprinted Polymer Electrospray Ionization Ion Mobility Spectrometry Zahra Badihi z Badihi@ch iut ac ir April 2011 Department of Chemistry Isfahan University of Technology Isfahan 84156 83111 IranDegree M Sc Lanquage FarsiSupervisers Dr Mohammad Taghi JafariEmail jafari@cc iut ac irAbstractIn this research a suitable molecularly imprinted polymer MIP as a solid phase extraction SPE sorbenthas been synthesized for the selective separation and cleaning up of salicylic acid from the biologicalsamples plasma and urine in negative mode electrospray ionization ion mobility spectrometry ESI IMS was used for the first time as a detection system In this study salicylic acid an acidic drug which can formintramolecular hydrogen bond between its two functional groups COOH and OH Although the process ofrecognition of MIP is similar to that of macrobiomoleculessuch as enzymes and antibodies MIP haveconsiderable advantages over biomoleculesas recognition systems because of their chemical and physicalstability Ion mobility spectrometry with low cost inexpensive maintenance and very fast analysis makes anattractive technique for determination of salicylic acid The imprinted polymer was prepared by non covalentmethod using salicylic acid as the template 4 vinyle pyridin 4 vpy as the functional monomer ethyleneglycol dimethacrylate EGDMA as cross linker agent and 2 2 azobis 2 isobutyronitrile AIBN asinitiator In this work Several factors affecting the performance of MIP such as water sample pH washingvolume raito and its speed were studied Sample pH was adjusted at pH 5 MIP sorbent was conditionedfirst with methanol and then with purified water 2 0 mL each solvent before being used for the first time The water sample 2 0 mL in each time was passed through the colmun with speed of 0 04 mL min Thesolid phase was then washed with 2 0 mL mixer water and methanol 95 5 to remove interference Theanalytes were then eluted with 20 mL methanol at speed of 0 04 mL min then 200 0 L of elution solventwas dried by a gentle stream of nitrogen gas The eluent was injected to ESI IMS injector In addition theseexperiments were compared with the non imprinted polymer NIP and the results indicated that NIP had nospecific affinity to adsorb salicylic acid The retention capacity of the prepared MIP sorbent was 2 6 mg g Under optimum conditions the proposed method with respect to other reported methods shows highsensitivity and selectivity with detection limit of 0 008 g mL The linear dynamic range of about 2 ordersof magnitude from 0 02 to 2 00 g mL was obtained for this compound and the relative standard deviationswere under 6 good precision Also the proposed method was used to analyze various real samples so Once the drug spiked human plasma and urine Once again Someone volunteers to take medication and hisurine and plasma were analyzed and the obtained results confirmed the capability of ESI IMS for detection ofsalicylic acid Recoveries were obtained as higher as 84 to 99 Key Words molecularly imprinted polymer Electrospray ionization ion mobilityspectrometry salicylic acid drug

بديهي، زهرا

استخراج گزينشي و اندازه گيري داروي ساليسيليك اسيد در پلاسما و ادرار انسان به كمك روش پليمر حكاكي شده مولكولي - طيف سنج تحرك يوني با منبع يونيزاسيون الكترواسپري

جاذب پليمري حكاكي شده با مولكول , نمونه هاي بيولوژيكي