پديد آورنده :
عمراني، نازگل
عنوان :
القاي موتاسيون با موتاژن هاي شيميايي و اشعه گاما و شناسايي موتانت هاي مفيد در كلزا
گرايش تحصيلي :
اصلاح نباتات
محل تحصيل :
اصفهان: دانشگاه صنعتي اصفهان، دانشكده كشاورزي
صفحه شمار :
بيست و يك،261ص.: مصور،جدول،نمودار
يادداشت :
ص.ع.به فارسي و انگليسي
استاد راهنما :
احمد ارزاني، قدرت ا...سعيدي
استاد مشاور :
محمد حسين فتوكيان
توصيفگر ها :
جهش , تنوع ژنتيكي
تاريخ نمايه سازي :
19/10/90
استاد داور :
بدرالدين ابراهيم سيد طباطبائي، منصور اميدي، غلامرضا بلالي
كد ايرانداك :
ID410 دكتري
چكيده فارسي :
به فارسي و انگليسي: قابل رويت در نسخه ديجيتالي
چكيده انگليسي :
283 Mutation Induction by Chemical Mutagens and Gamma Radiation and Detection of Useful Mutants in Canola Seyedeh Nazgol Emrani E mail address nazgol 6532@yahoo com Date of Submission November 26 2011 Department of Agronomy and Plant Breeding Isfahan University of Technology Isfahan 84156 83111 Iran1st Supervisor s Name A Arzani 1 E mail address a arzani@cc iut ac ir2st Supervisor s Name G Saeidi E mail address gsaeidi@cc iut ac ir1st Advisor s Name M H Fotokian 2 E mail address Fotokian@yahoo comDepartment Graduate Program Coordinator A RiasiAbstractIn order to induce and explore genetic variation in agronomic and quality traits bychemical mutagens and gamma rays in canola the following experiments wereconducted 1 determination of optimum dosage of chemical mutagens 2 fieldevaluation of M1 to M3 generations 3 assessment of genetic diversity of M2 lines usingmicrosatellite markers and 4 screening of mutant plants via TILLING Based on LD50criterion the 0 8 percent ethyl methanesulfonate for 6 hrs 12 mM N nitroso N ethylurea 6 mM sodium azide and 9 mM N nitroso N methylurea for 8 hrs wereconsidered as optimum treatment conditions The highest genetic diversity was achievedin sodium azide treatment Treatment with 1000 and 800 Gy dosages of gamma rayswere considered as the best treatment conditions for improving agronomic traits alongwith seed quality traits in RGS003 and Sarigol respectively Cluster analysis accordingto data derived from evaluation of genetic diversity using SSR markers grouped M2mutant lines in a completely separated group from control Screening of 750 M2 mutantplants using TILLING led to identification of 12 mutations in BnDGAT1 and 9mutations in BnIND The results of this study imply the potential of mutations toenhance the genetic variation available to canola breeders Key words mutation chemical mutagens gamma rays genetic diversity canola Department of Agronomy and Plant Breeding College of Agriculture Isfahan University ofTechnology Isfahan 84156 83111 Iran Department of Agronomy and Plant Breeding College of Agricultural Sciences Shahed University Tehran Iran
استاد راهنما :
احمد ارزاني، قدرت ا...سعيدي
استاد مشاور :
محمد حسين فتوكيان
استاد داور :
بدرالدين ابراهيم سيد طباطبائي، منصور اميدي، غلامرضا بلالي
