6570

6135

كارشناسي ارشد

بوم شناسي آبزيان شيلاتي

اصفهان: دانشگاه صنعتي اصفهان، دانشكده منابع طبيعي

1390

ده، 62ص.: مصور، جدول، عكس، نمودار

ص.ع. به فارسي و انگليسي

سالار درافشان

احمد قاسمي

19/1/91

احمد قاسمي، منصوره ملكيان، اميدوار فرهاديان

1396/10/10

كتابنامه

منابع طبيعي

مهندسي منابع طبيعي

ID6135

به فارسي و انگليسي: قابل رويت در نسخه ديجيتالي

Genetic Diversity of Alburnus mossulensis Heckel 1843 in Tigris Basin Using Microsatellite Markers Zahra Shafiei Falavarjani zahrashafei iut@yahoo com February 25 2012 Department of Natural Resources Isfahan University of Technology Isfahan 84156 83111 IranDegree M Sc Language FarsiDr Salar Dorafshan sdorafshan@cc iut ac irDr Yazdan Keivany keivany@cc iut ac irAbstractThe Mosul bleak Alburnus mossulensis Heckel 1843 in Iran is widely distributed in Fars Isfahan Tigris andBushehr basins Considering the importance of the genetic variation and population structure for managementand conservation of species especially native fish the genetic diversity of Mosul bleak was investigated usingmolecular techniques In this study 30 specimens of Mosul bleak were collected from each locality Konjancham Kashgan Gamasiab Doroud and Dopolan rivers and 25 specimens from Davoud Arrab River For molecularstudy DNA was extracted using ammonium acetate method and Takara kit from bodies tissues collected fromeach stations The DNA quality was examined by electrophoresis on 1 agaros gel and painted with ethidiumbromide PCR amplification was performed using four pairs of microsatellite primers CypG24 BL1 2b BL1 98and Rser10 at annealing temperature 56 C PCR products electrophoresed on 12 achrylamid gel and paintedwith silver nitrate Calculations were done using PowerMarker ver 3 0 PopGene ver 3 2 ARLEQUIN ver 3 11 MEGA ver 4 and NTSYS ver 2 02 software Number of the observed alleles at all loci ranged from 8 BL1 98 to14 CypG24 and averaged 10 75 alleles per locus Allele sizes at CypG24 BL1 2b BL1 98 and Rser10 loci werein the range of 138 215 141 180 272 300 176 240 bps respectively The mean of polymorphism informationcontent PIC at all loci was 0 835 Among the pair four markers BL1 98 was the lowest polymorphic andCypG24 was the highest polymorphic one The expected heterozygosity ranged from 0 79 in Davoud Arrab Riverand 0 84 in Konjancham and Kashgan rivers and averaged 0 825 The observed heterozygosity ranged from 0 69in Konjancham River and 0 81in Kashgan River and averaged 0 75 Significant deviations from Hardy WeinbergEquilibrium expectation were found at BL1 98 BL1 2b and CypG24 in all population and Rser10 in DavoudArrab population p 0001 The analysis of molecular variance AMOVA indicated that the percent of varianceamong populations Among individuals within populations and within individuals were 2 01 15 6 and 82 4respectively Only a small proportion 2 01 of the variation was attributed to differences between thepopulations and large proportion of the variation was attributed to differences within individuals The overallvalue of FST values between populations and basins were 0 02 and 0 0071 respectively The genetic similarity anddistance index came in the range of 0 696 0 894 and 0 111 0 34 respectively The level of genetic variability washigh in the studied populations but comparison of mean expected heterozygosity values revealed that these locidid not show significant differences between the populations Although no significant genetic variation betweenthe populations of bleak existed significant genetic differentiation was found between them p 005 The lowgenetic structure could be due to high gene flow For better understanding of population structure of Mosul bleak population structure studies using other molecular techniques from all distributed areas followed by ecologicaland or biological researches should be considered Keywords Mosul bleak Alburnus mossulensis microsatellite population variety geneticdifferentiation

سالار درافشان

احمد قاسمي

احمد قاسمي، منصوره ملكيان، اميدوار فرهاديان