بررسي تنوع ژنتيكي بين و درون جمعيت در ارقام، لاين ها و توده هاي گلرنگ با استفاده از نشانگرهاي مولكولي EST-SSR

چكيده انگليسي :

۶۲ Assessment of Inter and Intra Population Genetic Variation in Cultivars Lines and Populations of Safflower using EST SSR Molecular Markers Mohammad Barati Moh barati@yahoo com Department of Agronomy and Plant Breeding Isfahan University of Technology Isfahan 84156 83111 IranDegree M Sc Superviser Ahmad Arzani a arzani@cc iut ac irAbstractMicrosatellite SSR markers originated from the expressed sequence tag EST region of genome arefunctionally more informative than the anonymous molecular markers for study of genetic diversity Withdevelopment of the EST SSR markers in safflower their utilization in genetic and breeding studies ofsafflower is now possible The present study was aimed at estimating genetic variation in safflower usingcultivars lines cultivated and wild populations with EST SSR molecular markers Intra population geneticvariation and genetic purity of safflower genotypes were also evaluated Forty eight safflower genotypesincluding 42 cultivated genotypes belong to Carthamus tinctorius and 6 wild accessions belong to C oxyacanthus and C lanatus species were used The results indicated 42 of 109 EST SSR primers pairsproduced polymorphic bands with clear banding patterns A total of 145 bands ranged from 100 bp to 500bp were developed and scored An average of 3 43 alleles per locus was detected Dice similaritycoefficients had a range of 0 26 to 0 98 indicating high genetic variation among genotypes Dice similaritycoefficients for the cultivated genotypes ranged from 0 68 to 0 98 High transferability of EST SSR markersobserved in present study makes them useful in cross species genetic studies and basic evolutionaryapplications The results of the present study showed that C oxyacanthus accessions are more closelyrelated to cultivated species than C lanatus Based on cluster and principal coordinate analyses safflowergenotypes were grouped into three main clusters C lanatus C oxyacanthus and C tinctorius The 42cultivated safflower genotypes divided into 4 subgroups The subgroup one included 16 genotypes most ofwhich obtained from Europe America such as PI 537636 PI 258417 PI 198844 landraces and Hartman Gila Yinic cultivars The subgroup two comprised 18 accessions originated from Palestine Egypt andMexico as well as 15 Iranian cultivars lines and landraces Most Iranian accessions were belonged to thissubgroup The subgroup three contained 7 genotypes including a Syrian landrace and 6 Iranian genotypes Sina cultivar and 5 Iranian landraces PI 506426 landrace obtained from China separated solely intosubgroup four These results indicated that in most cases the cultivated safflowers divided into thesubgroups based on their country of origin and genetic background However there was slightinconsistency between country of origin and subgroup of some genotypes Results of analysis of molecularvariance showed a significant difference between Iranian and exotic genotypes Although the fixationindex value FST 0 05 observed in the present study suggests a relatively low level of geneticdifferentiation between the two groups The results of population genetic structure of four safflowerpopulation with four SSR primers indicated that Isfahan W and C4110 populations had the highest andlowest values of observed and expected heterozygosity gene diversity respectively The fixation index FST values indicating the differentiation between subpopulations ranged from 0 21 to 052 with anaverage of 0 39 which shows existence of high differentiation between used populations A high value ofgene diversity was observed in Arak landrace indicating the potential of landraces as origin of selection toproduce cultivars and breeding lines In conclusion these markers were effective for evaluation of geneticvariation and population genetic analysis in the safflower species Key words Safflower Genetic variation Express sequence tag EST Simple sequencerepeat SSR Carthamus

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بررسي تنوع ژنتيكي بين و درون جمعيت در ارقام، لاين ها و توده هاي گلرنگ با استفاده از نشانگرهاي مولكولي EST-SSR

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