پديد آورنده :
شهبازي، اسلام
عنوان :
ايجاد لاين هاي دابل ها هاپلوئيد در كلزا با استفاده از كشت ميكروسپور و ارزيابي آنها براي مقاومت به تنش شوري
گرايش تحصيلي :
اصلاح نباتات﴿ژنتيك بيومتري﴾
محل تحصيل :
اصفهان: دانشگاه صنعتي اصفهان، دانشكده كشاورزي
صفحه شمار :
پانزده،191ص.: جدول،نمودار
يادداشت :
ص.ع.به فارسي و انگليسي
استاد راهنما :
احمد ارزاني، قدرت ا... سعيدي
استاد مشاور :
بدرالدين ابراهيم سيدطباطبائي، حسين زينلي
توصيفگر ها :
كشت درون شيشه اي
تاريخ نمايه سازي :
27/1/91
استاد داور :
آقافخرميرلوحي،بهروز شيران،سعدا...هوشمند
كد ايرانداك :
ID425 دكتري
چكيده فارسي :
به فارسي و انگليسي: قابل رويت در نسخه ديجيتالي
چكيده انگليسي :
Production of canola double haploid lines by microspore culture and evaluation of their tolerance to salinity stress Eslam Shahbazi E mail address es shahbazi@yahoo com Date of Submission January 16 2012 Department of Agronomy and Plant Breeding college of AgricultureIsfahan University of Technology Isfahan 84156 83111 Iran1st Supervisor s Name A Arzani a arzani@cc iut ac ir2nd Supervisor s Name G Saeidi gsaeidi@cc iut ac ir1st Advisor s Name B E Sayed Tabatabaei sayedt@cc iut ac ir2nd Advisor s Name H Zeinali hoszeinali@yahoo comDepartment Graduate Program Coordinator A RiasiAbstract The present study aimed to improve the efficiency of microspore culture for production ofdoubled haploid lines DHs and to evaluate the resultant DH lines for salinity toleranceunder in vitro and greenhouse conditions Four genotypes including H1 and H2 hybrids andRGs003 and Okapi cultivars were used Results showed that H1 and were more responsive tomicrospore culture and addition of 13 sucrose to the NLN induction medium performedbetter than other concentrations of 10 and 17 for embryogenesis Adding 0 1 mgl 1 GA3 into B2 medium caused a positive effect on plant regeneration Results of in vitro salinityassessment revealed the significant variation among DH lines for salinity tolerance atcellular level with DH lines 13 78 25 68 16 and 21 identified as being salt tolerant Cluster analysis divided 16 13 21 25 68 58 and 36 lines in to a single group possessingthe highest dry weight fresh weight plant height K and Ca2 concentration K Na ratioand Ca2 Na ratio and regarded as being salt tolerant at greenhouse conditions Inconclusion our data suggest that DH lines 13 and 16 were the most salt tolerant and DHlines 24 and 97 were the most salt sensitive Further studies are needed to better elucidate thephysiological mechanism and genetic basis underlying salinity tolerance in these lines andultimately their utilization in canola breeding program can be recommended Key words canola microspore culture doubled haploid salinity In Vitro culture Department of Agronomy and Plant Breeding Faculty of Agriculture Isfahan University of Technology Isfahan 84156 83111 Iran Research Center of Agriculture and Natural Resources of Esfahan Isfahan Iran
استاد راهنما :
احمد ارزاني، قدرت ا... سعيدي
استاد مشاور :
بدرالدين ابراهيم سيدطباطبائي، حسين زينلي
استاد داور :
آقافخرميرلوحي،بهروز شيران،سعدا...هوشمند
