6649

6200

كارشناسي ارشد

علوم دامي

اصفهان: دانشگاه صنعتي اصهان، دانشكده كشاورزي

1390

يازده، 48ص.: مصور، جدول، نمودار

ص.ع. به فارسي و انگليسي

محمدعلي ادريس، سعيد انصاري مهياري

محمد خوروش، بدرالدين ابراهيم سيد طباطبائي

12/2/91

غلامرضا قرباني، مجيد طالبي

1396/10/06

كتابنامه

كشاورزي

مهندسي كشاورزي

ID6200

به فارسي و انگليسي: قابل رويت در نسخه ديجيتالي

57 Evaluation of Relationship Between the ABCG2 Gene with Production Traits in Holstein Dairy Cattle Mahmud Rostami Engineer rostami@yahoo com Date of Submission 2011 12 27 Department of Animal Science Isfahan University of Technology Isfahan 84156 83111 IranDegree M Sc Language FarsiSupervisor M A Edriss edriss@cc iut ac irAbstract ABCG2 protein or Breast Cancer Resistant Protein Bcrp located in membrane of mammary glandsalveolar epithelial cells is one of the most important active transport proteins and responsible for passing offat from blood to milk It is encoded by ABCG2 Bcrp These transport fat are very important forveterinary medicine Single nucleotide polymorphism which is caused by changing amino acid Y581S inABCG2 gene that encodes Bcrp affect milk yield and milk properties in Israeli Holstein In the case ofbeing nucleotide C there will be restriction site for the restriction enzyme PstI and as a result alleles A andC will be recognizable as bands with length 292bp and 268bp respectively To hnvestigate thispolymorphism and its relationship with milk production traits blood sample was randomly taken from 408Holstein cattle in 5 industrial dairy farms located in Isfahan province that had records for milk productionand its composition for first parity Genotyping was done by PCR RFLP technique After extracting DNA determining the quality and quantity of extracted DNA amplification and digestion by restriction enzyme digestion product was observed on 2 5 agarose gel Frequencies of all A and C werw 0 97 and 0 03respectively totally Frequency of genotypes AA and AC werw also 0 94 and 0 06 respectively On theother hand CC homozygous had not been found in investigated animals All of them were in Hardy Weinberg equilibrium Being in equilibrium can be owing to selecting animals according not to ABCG2gene Genetic analysis of the population was done by the software PopGen32 Resulted indices indicateshigh diversity of this polymorphism and high inbreeding and genetic relationship in the studied population To identify the effect of genotype on production traits the software SAS procedure GLM was used In theanalysis model the effects genotype and combined effect of herd year and season of parturition wereconsidered as fixed effects and days in lactation and open days were considered as covariables Genotypehad significant effect on fat and the breeding value of milk production P 0 001 Individuals withgenotype AC had statistically more milk fat percentage and genotype AA breeding value of milkproduction This finding can be a reason that the effect of this polymorphism is additive For trait milk fatpercentage the average effect of substitution of allele C was significant P 0 001 but dominance effectfor the studied traits was not significant P 0 05 which indicates the effect of the polymorphism asadditive Regarding the relationship between the polymorphism and traits milk fat percentage and thebreeding value of milk production in the studied population the polymorphism can be used is selection forthese traits Key words ABCG2 Gene Holstein Cattle Genotype Polymorphism

محمدعلي ادريس، سعيد انصاري مهياري

محمد خوروش، بدرالدين ابراهيم سيد طباطبائي

غلامرضا قرباني، مجيد طالبي