پديد آورنده :
قنبري باغنوئي، سجاد
عنوان :
ارتباط بين پلي مورفيسم ژن گيرنده لپتين با صفات توليدي در گاو هلشتاين
مقطع تحصيلي :
كارشناسي ارشد
محل تحصيل :
اصفهان: دانشگاه صنعتي اصفهان، دانشكده كشاورزي
صفحه شمار :
دوازده، 80ص.: مصور، جدول
يادداشت :
ص.ع.به فارسي و انگليسي
استاد راهنما :
سعيد انصاري مهياري، محمد علي ادريس
استاد مشاور :
بدرالدين ابراهيم سيد طباطبائي
توصيفگر ها :
چند شكلي , صفات عملكردي , گاوهاي شيري
تاريخ نمايه سازي :
12/2/91
استاد داور :
حميدرضا رحماني، آقا فخر مير لوحي
تاريخ ورود اطلاعات :
1396/10/06
چكيده فارسي :
به فارسي و انگليسي: قابل رويت در نسخه ديجيتالي
چكيده انگليسي :
Association Between Polymorphism of Leptin Receptor Gene with Productive Traits in Holstein Dairy Cow Sajad Ghanbari Baghenoey S ghanbari@ag iut ac ir Date of submission December 2011 Department of Animal Science Isfahan University of Technology Isfahan 84156 83111 Iran Degree M Sc Language Farsi Supervisors Saeid Ansari Mahyari s ansari@cc iut ac ir Mohammad Ali Edriss edriss@cc iut ac ir Abstract During the last decades strong genetic progress achieved in milk yield led to decreased reproductiveperformance in dairy cows Declined fertility is believed to result from the negative energy balance NEB caused by high milk production combined with limited feed intake in early lactation Recently much effort has been devoted to understand the role of the leptin and its receptor in regulating food intakeand reproduction in ruminants Leptin is secreted by adipose tissues and acts especially through itsreceptor on the hypothalamus the center of energy homeostasis as well as on ovarian follicular cells onplacenta and mammary glands Genes encoding leptin LEP and leptin receptor LEPR are located inbovine chromosomes 4 and 3 respectively Inside the LEPR gene discover a missense mutation T945M The C to T SNP is located in exon 20 at position 115 and causes the threonine to methionine amino acidsubstitution in the intracellular domain of the LEPR b isoform at residue 945 It might be responsible forthe efficiency of the signaling pathway in cells and as a consequence for the modulation of the leptineffects The objectives of this study were to estimate the allelic and genotypic frequencies of leptinreceptor gene in Holstein cows and also evaluating the association between gene polymorphism and themilk and reproductive traits For this purpose blood samples were collected from 395 cows of fiveHolstein dairy herds in Isfahan province Number of Samples varied from 64 to 96 among the herds DNA was extracted using salting out procedure The DNA quality and quantity were determined usingelectrophoresis The 400 bp fragment of the gene was amplified by specific primers and Thermocycler PCR products were detected by electrophoresis on 1 TAE agarose gel Amplified fragment digested viaTaqI restriction enzyme in 8 to 14 hours at 37 C The genotype Frequencies of BB PB and PP were 0 11 0 49 and 0 40 respectively The frequencies of alleles P and B were 0 648 and 0 352 respectively Thegenotypes were distributed according to the Hardy Weinberg equilibrium Shannon s and Nei s diversityindices showed that gene diversity is the same in all herds The analysis by GLM procedure SASsoftware did not reveal any significant effect of the T945M polymorphism on adjusted 305 day milk fatpercentage protein percentages lactation length and conception rate P 0 05 Key words Leptin receptor gene Polymorphism Performance traits Dairy cows
استاد راهنما :
سعيد انصاري مهياري، محمد علي ادريس
استاد مشاور :
بدرالدين ابراهيم سيد طباطبائي
استاد داور :
حميدرضا رحماني، آقا فخر مير لوحي
