6667

6218

كارشناسي ارشد

مرتعداري

اصفهان: دانشگاه صنعتي اصفهان، دانشكده منابع طبيعي

1390

يازده، 90ص.: مصور، جدول، نمودار

ص.ع. به فارسي و انگليسي

محمدرضا وهابي، محمد فضيلتي

كامران قائدي

13/2/91

مصطفي تركش اصفهاني، منصوره ملكيان

1396/10/06

كتابنامه

منابع طبيعي

مهندسي منابع طبيعي

ID6218

به فارسي و انگليسي: قابل رويت در نسخه ديجيتالي

Genetic Comparison of Astragalus gossypinus Fisher Populations in the Western Habitats of Isfahan Province Zahra dehdashtian Date of Submission 2012 02 06 Department of Natural Resources Isfahan University of Technology Isfahan 84156 83111 Iran Degree M Sc Language FarsiSupervisor vahabi @cc iut ac irSupervisor fazilati @cc iut ac irAbstract Iran with a diverse climate and rich herbal diversity is one of the richest countries in natural geneticresources Iran is also the main origin and one of the most important centers of Astragal species diversity Astragalus gossypinus Fischer which produce gum tragacanth belongs to papilionacae and mainly spreadin Asia This species distributed in Iran Iraq Lebanon and syria The most important habitats of gumproducer Astragalus species are at montain ranges of IranoTooran region especially Alborz and Zagrosheights Isfahan province have maximum number of Astragal species in Iran 154 species Astragalushabitats in Isfahan province cover 31 1 percent of its area Astragalus gossypinus mostly studiedecologically and morphologically In this study genetic attributes of Astragalus gossypinus populations inwestern rangelands of Isfahan province has been investigated and compared The results of such studiescould use in interbreeding production and increase of Astragalus individuals with high quality gumtragacanth reclamation of destructed habitats or amplification of Astragalus habitats To do so Astragalusgossypinus Fischer samples collected from six rangeland sites from its habitats in Isfahan province including Rahmat abad Khansar Ghale jamal Golpayegan Betlijeh Hendokosh Fereidan mousaabad Tiran Karvan and Tang bidakan Lenjan Genomic DNA was extracted using CTAB method andquality of extracted DNA was checked on electrophorus gel DNA density measured by spektrofotometrymethod After assuring the quality of DNA DNA fragment of CNGC4 gene was amplified and the PCRproduct was checked on agaroze gel The resulted product was sequenced and aligned using CLC MainWorkbench5 program For amplification two primers designed by Oligo6 software The PCR reaction hasbeen done on a part of CNGC4 gene The results of gene allignment of CNGC4 showed a genetic similaritybetween Astragalus gossypinus populations Regarding genetic similarity Astragalus gossypinuspopulations considered as one genetype The genom of Astragalus gossypinus also compared with otherAstragal species The results of cluster analysis and UPGMA method indicated the existence of three majorgroups between Astragal species According to these results and previous studies about quantity and qualityof gum tragacanth products of this species it can be concluded that since Astragalus gossypinus has anarrow ecological niche and resistance range the difference in quantitative and qualitative attributes of itsgum tragacanth in different habitats are caused from environmental conditions affecting physiological andfunctional life of Astragalus gossypinus and may not be attributed to genetic diversity of this species Keywords Astragalus gossypinus Genetic diversity DNA PCR Allignment Cluster analysis habitat Isfahanprovince

محمدرضا وهابي، محمد فضيلتي

كامران قائدي

مصطفي تركش اصفهاني، منصوره ملكيان