تنوع ژنتيكي گله هاي پرورشي قزل آلاي رنگين كمان ﴿oncorhynchus mykiss﴾ با استفاده از نشانگر ريز ماهواره

چكيده انگليسي :

Genetic Diversity of Farmed Rainbow Trout Oncorhynchus mykiss Stocks Using Microsatellite Marker Abulfath Alipoor Alipoor64@gmail com December 6 2011 Department of Natural Resources Isfahan University of Technology Isfahan 84156 83111 IranDegree M Sc Language FarsiDr Salar Dorafshan sdorafshan@cc iut ac irAbstractRainbow trout Oncorhynchus mykiss of Salmonidae family is one of the most important fishfarmed in Iran Considering the importance of genetic variation and population structure formanagement and broodstock selection for replication competent in the farms the genetic diversityof rainbow trout was investiged using molecular techniques In this study 30 specimens of rainbowtrout were collected from each of four populations Iranian American Spanish and French Formolecular study DNA was extracted using ammonium acetate method from fin clips and or part ofbodies The DNA quality was examined by electrophoresis on 1 agaros gel and painted withethidium bromide PCR amplification was performed using four pairs of microsatellite primers OMY77 OMY325 OMM1329 and OMM1332 at annealing temperatures of 58 5 62 5 PCRproducts were electrophoresed on 12 achrylamid gel and stained with silver nitrate Calculationswas done using GenAlex 6 software All loci were polymorphic Number of observed alleles werein the range of 8 16 Average number of observed alleles in Iranian American Spanish and Frenchpopulations were 10 75 11 11 5 and 10 to respectively The number of effective alleles were inthe range of 5 40 11 53 Average number of effective alleles in Iranian American Spanish andFrench populations were 7 43 8 03 8 65 and 7 19 respectively Allele sizes at OMY77 OMY325 OMM1329 and OMM1332 loci were in the range of 102 178 100 150 122 198 172 204 bpsrespectively The means of observed heterozygosities in Iranian American Spanish and Frenchpopulations were 0 591 0 591 0 633 and 0 566 to respectively The mean of expectedheterozygosity for Iranian American Spanish and French populations were 0 861 0 868 0 879and 0 854 respectively Results showed deviation from Hardy Weinberg equilibrium at fourteenout of sixteen studied loci Increase of observed heterozygosity towards expected heterozygosity atOMY325 loci in Iranian OMY325 in American OMY325 in Spanish and French populations low 7 genetic segregation between population high 93 genetic diversity within population andhigh 16 07 gene flow were observed Genetic identity and genetic distance between all populationwere in the range of 0 727 0 847 and 0 166 0 319 respectively A tree diagram of the fourpopulations based on Nei 1972 1978 genetic distance and UPGMA method was performed Based on the results American Spanish and French populations were put in a same branch whileIranian population was placed in separate branch The FST was measured as 0 023 and FIS inIranian American Spanish and French populations were 0 329 0 334 0 295 and 0 343respectively As regards to separation of Iranian population from other populations it can be saidthat for implementation of conflux program of two populations for hybridization the best choice isconflux Iranian population with one of the other populations The genetic diversity value ofSpanish population was more than others For better understanding of genetic structure ofcultivated rainbow trout as one of the most important cultivated species in Iran study on allcultivated stocks using higher number of microsatellite loci or other molecular markers is highlyrecommend Keyword Rainbow trout Oncorhynchus mykiss Microsatellite Genetic diversity Geneticvariation

عليپور، ابوالفتح

تنوع ژنتيكي گله هاي پرورشي قزل آلاي رنگين كمان ﴿oncorhynchus mykiss﴾ با استفاده از نشانگر ريز ماهواره

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