6708

6259

كارشناسي ارشد

شيمي معدني

اصفهان: دانشگاه صنعتي اصفهان، دانشكده شيمي

1390

سيزده، 82ص.: مصور، جدول، نمودار

ص.ع. به فارسي و انگليسي

حسن حدادزاده

مونا سليمي

23/2/91

كاظم كرمي، حسين چيني فروشان

1396/10/06

كتابنامه

شيمي

شيمي

ID6259

به فارسي و انگليسي: قابل رويت در نسخه ديجيتالي

Ciprofloxacin Copper II complexes the study of interaction of Cu cip 2 OH 2 with DNA Tahereh Ahmadi bagheri t ahmadibagheri@ch iut ac ir Date of Submission 2011 12 10 Department of Chemistry Isfahan University of Technology Isfahan 84156 83111 IranDegree M S Language FarsiH Hadadzadeh Assoc Prof hadad@cc iut ac ir Abstract In this thesis three mononuclear complexes of Cu II Cu cip 2 OH 2 2CH3OH 6H2O Cu cip bpy H2O 2 PF6 and Cu cip phen H2O 2 PF6 H2O where cip is 1 cyclopropyl 6 fluoro 4 oxo 7 1 piperazinyl 1 4 dihydroquinoline 3 carboxylic acid have been prepared and characterized by elementalanalysis FT IR and UV vis spectroscopies Solid state structure of Cu cip 2 OH 2 2CH3OH 6H2O complexwas determined by single crystal X ray crystallography Blue crystals of Cu cip 2 OH 2 were grown byaceton diffusion into an aqueous solution of the complex This complex crystallized in triclinic crystalsystem with space group of P 1 with the following unit cell parameters a 9 376 4 b 9 576 5 c 11 200 5 87 46 80 67 86 22 Z 1 and V 3 989 9 8 Single crystal structureshowed that the coordination geometry around the Cu II was a distorted octahedron with bite angles of86 73 6 for the cip ligands The two main quinolone rings of the cip ligands are nearly coplanar The cipligand is coordinated to Cu II ions through one carboxylate and the exocyclic carbonyl oxygen atoms Thecip ligand exists in its zwitterionic form due to the presence piperazine basic nitrogen and the deprotonatedcarboxylic group The FT IR spectra of these complexes showed that carbonyl and carboxylate bands are shifted and theirintensity are reduced in comparison to free cip ligand Electronic spectra of these complexes were taken inwater The intense absorption bands seen in the UV region are assigned to ligand centered transitions and aweak absorption band seen in the visible region is assigned to d d transition The interaction of the Cu cip 2 OH 2 complex with DNA was investigated by UV vis and fluorescenceSpectroscopies cyclic voltammetry and gel electrophoresis Absorption spectral titration of complex withfish DNA indicate hypochromism and red shift in their intraligand transfer band suggesting that the complexbinds to DNA The magnitude of the binding constant Kb was obtained from absorption spectral titration All the results showed the majority of interaction mode between the complex and DNA was intercalation DNA cleavage experiments showed that the complex induce cleavage of pEGFP N1 plasmid DNA An in vitro cytotoxicity study of the Cu cip 2 OH 2 complex on human breast adenocarcinoma MCF7 cell line by an MTT assay indicated that the complex may have anticancer potency with a cytotoxicity valueof 9 3 1 4 Key wordQuinolone antibiotics Ciprofloxacin complexes DNA binding DNA cleavage cytotoxicity activity

حسن حدادزاده

مونا سليمي

كاظم كرمي، حسين چيني فروشان