پديد آورنده :
عبدي، ريحانه
عنوان :
بهينه سازي توليد اگزوپلي ساكاريد و بررسي تاثير آنزيم هاي مصرف كننده ساكارز برتوليد آن در گونه هاي لاكتوباسيلوس
گرايش تحصيلي :
علوم و صنايع غذايي
محل تحصيل :
اصفهان: دانشگاه صنعتي اصفهان، دانشكده كشاورزي
صفحه شمار :
چهارده،139ص.: مصور،جدول،نمودار
يادداشت :
ص.ع.به فارسي و انگليسي
استاد راهنما :
محمود شيخ زين الدين، صبيحه سليمانيان زاد
استاد مشاور :
مايكل گنزل، محمد شاهدي
توصيفگر ها :
لاكتوباسيلوس بولگاريكوس SZ2 , لاكتوباسيلوس روترابي LTH5448
تاريخ نمايه سازي :
6/3/91
استاد داور :
محمدرضا احساني، محمد رباني، علي نصيرپور
كد ايرانداك :
ID426 دكتري
چكيده فارسي :
به فارسي و انگليسي: قابل رويت در نسخه ديجيتالي
چكيده انگليسي :
Effects of Sucrose metabolizing Enzymes on and Optimization of Exopolysaccharide Production by Lactobacillus Species Reyhane Abdi reihane abdi@ag iut ac ir reihaneh@ualberta ca 2 29 2012 Department of Food Science and Technology Isfahan University of Technology Isfahan 84156 83111 IranSupervisorsDr M Sheikh Zeinoddin zeinodin@cc iut ac irDr S Soleimanian Zad soleiman@cc iut ac irAdvisorsDr M G nzle michael ganzle@ualberta caDr M Shahedi shahedim@cc iut ac irDepartment of Graduate Program CoordinatorDr A Riasi Departmetn of Food Science and Technology Isfahan University of Technology Isfahan 84156 83111 Iran Department of Agricultural Food and Nutritional Science University of Alberta Edmonton T6G 2P5 CanadaAbstractExopolysaccharide production by Lactobacillus bulgaricus SZ2 was optimized inmodified MRS M1 MRS Maximum EPS production was 74 3 2 mg L and theoptimized values of the three variables predicted for maximum EPS production includeda temperature of 38 7 C Bacto casitone and glucose concentrations of 24 5 and 29 6g L respectively The kinetics of EPS formation and growth were monitored in M1 MRS skimmed milk SM skimmed milk plus sucrose Suc SM and skimmed milkcontaining Bacto casitone and yeast nitrogen base BY SM EPS production in all themedia seemed to be growth related The EPS cell ratios were determined to be 3 12 10 10 1 43 10 10 4 42 10 11 and 3 16 10 11 mg cell in Suc SM SM M1 MRS and BY SM respectively clearly indicating the greater effect of C N ratio The effects offructosyltransferase and sucrose phosphorylase genes inactivation on the sucrosemetabolism in L reuteri LTH5448 strains were compared Inactivation of ftfA gene hadno influence on growth and only a minor impact on sucrose turnover implyingfrucotsyltransferase contributes to sucrose utilization and sucrose phosphorylasemediates sucrose metabolism in the absence of frucotsyltransferase activity In contrastto the wild type and scrP strains ftfA mutant did not produced anyhomoppolysaccharide and fructooligosaccharides indicating frucotsyltransferase is theonly responsible enzyme for EPS and fructooligosacharide formation in L reuteriLTH5448 Keywords Exopolysaccharide L bulgaricus SZ2 L reuteri LTH5448 fructosyltransferase and sucrosephosphorylase
استاد راهنما :
محمود شيخ زين الدين، صبيحه سليمانيان زاد
استاد مشاور :
مايكل گنزل، محمد شاهدي
استاد داور :
محمدرضا احساني، محمد رباني، علي نصيرپور
