استفاده از برخي ژن هاي كلروپلاستي دخيل در فتوسنتز و باركدهاي گياهي براي تجزيه و تحليل روابط فيلوژني انار

چكيده انگليسي :

Phylogenetic Analysis of Pomegranate Using Chloroplast Photosynthetic and Plant Barcode Genes Zahra Hajiahmadi z hajiahmadi1366@gmail com Date of submission January 8 2012 Department of Agricultural Biotechnology Isfahan University of Technology Isfahan 84156 83111 Iran Degree M Sc Language Farsi 1 B E Sayed Tabatabaei Professor Supervisor sayedt@cc iut ac ir 2 M Talebi Ph D Assist Professor Supervisor mtalebi@cc iut ac irAbstractPomegranate Punica granatum L is one of the oldest and most important horticultural plants in Iran Since Iranseems to be the origin of pomegranate precise detection of genetic relationships among cultivars using molecularDNA markers is necessary to improve future breeding programs Nowadays different molecular markers have beenused to determine the genetic diversity among some pomegranate cultivars such as RAPD AFLP and SSR Notwithstanding high morphological diversity among the cultivars low level of polymorphism detected using thesemethods which maybe attribute to the effects of climate conditions posttranscriptional modifications and cytoplasmgenes on morphological traits Chloroplast DNA has been used extensively to analyze plant phylogenies at differenttaxonomic levels because of its size organization and sequence conservation so study of pomegranate chloroplastgenome can be used to reveal phylogenetic relationships of this plant with others Therefore in the present researchwe used two chloroplast region named petA psaJ petA psbJ psbL psbF psbE petL petG tRNApro tRNAtrp psaJ and trnC trnD petN tRNAAsp psbM to investigate the genetic diversity of pomegranate In addition we used fourDNA barcodes psbA trnH ITS rbcL matK in order to study intergeneric relationships of pomegranate and alsointra and inter species variation of Myrtaceae Punicaceae and Lythraceae families Chloroplast DNAs wereextracted from the wild cultivated and ornamental pomegranate genotypes and a wild type of myrtle Myrtuscommunis L as the outgroup data of Myrtaceae These genes were amplified using designed primer pairs and thensequenced Analysis of psbE petL in petA psaJ revealed 1300 nucleotide with 4 98 genetic diversity amongstudied genotypes Calculating the average of K2P 0 03 P distance 0 028 and Pairwise distance 0 03 indicatedrelatively higher diversity compared with other parts of this region PetN in trnC trnD region has 95 nucleotide and1 03 gene diversity TrnC trnD region had low diversity in compare to petA psaJ region Therefore despite otherstudies in other plants this region had a low potential to evaluate diversity among pomegranate cultivars Results ofplant barcodes analysis showed that two region named ITS 0 012676 Coalescent depth 0 017417 and psbA trnH 0 00524 Coalescent depth 0 003526 can be used as complementary barcodes in Myrtaceae family and ITSregion 0 013953 Coalescent depth 0 026418 can be used for phylogenetic studies of Lythraceae In contrast results showed that psbA trnH 0 0165 provides the highest rates followed by ITS 0 006 in Punicaceaefamily probably complementary use of them can be solved classification problem at species level of this family Also the results of this study suggested that two regions named psbE petL and psbA trnH are more efficient todetermine intra species relationships of pomagranate According to the dendrogram of plant barcodes pomegranateis closer to Lythraceae than Myrtaceae Keywords Pomegranate Chloroplast petA psaJ trnC trnD matK ITS psbA trnH rbcL

حاجي احمدي، زهرا

استفاده از برخي ژن هاي كلروپلاستي دخيل در فتوسنتز و باركدهاي گياهي براي تجزيه و تحليل روابط فيلوژني انار

petA-psa.J , psbA , rbcL , matK