7180

6686

كارشناسي ارشد

شيمي تجزيه

اصفهان: دانشگاه صنعتي اصفهان، دانشكده شيمي

1390

سيزده، 83ص.: مصور، جدول، نمودار

ص.ع. به فارسي و انگليسي

تقي خياميان

8/8/91

محمدسراجي، حسن قاضي عسگر

1396/09/20

كتابنامه

شيمي

شيمي

ID6686

به فارسي و انگليسي: قابل رويت در نسخه ديجيتالي

Analysis of Octreotide and Oxytocin Using Liquid Liquid Electroextraction And ion mobility spectrometry with electro spray ionization source Elham zarinkhat e zarinkhat@ch iut ac ir Data of submission 13 March 2012 Department of Chemistry Isfahan University of Technology Isfahan 84156 83111 IranDegree M Sc Language FarsiProf T Khayamian Email Taghi@cc iut ac irAbstractIn this thesis the quantitative analysis of two peptide drugs consisting of octreotide and oxytocin carried out using electrospray ion mobility spectrometry for first time Ion mobility spectra were obtained for bothdrugs In the spectrum related to each drug two peaks appeared Reduced mobility values of 1 171 and0 651 cm2V 1s 1 for octreotide and 1 231 and 0 655 cm2V 1s 1 for oxytocin were obtained For quantitativeanalysis the presence of acetic acid and water on signal intensity of these two substances was studied andpercent of water acid optimized value was obtained At first the quantitative analysis of octreotid in themixture was done that was as so 1 29 70 acid water methanol In each analysis ion mobility spectrumwas recorded and integral of area below the peaks when signal intensity was maximum was registered asinstrument response In this case linear range was 0 05 1 micro molar and detection limit was 0 007 micromolar Relative standard deviation was 2 7 Oxytocin too was analysis similarly Linear range anddetection limit 0 3 10 and 0 04 micro molar were obtained respectively Relative standard deviation was2 7 In this thesis the liquid liquid electro extraction method was used for the sample preparation whichis independent of the peptide s isoelectric point hydrophilicity or hydrophilicity In order to a cell Glasstube with a diameter of 15 mm and length 108 mm and a conical end designed and made A rod electrodemade of platinum with a diameter of 0 5 mm as the cathode was welded to the end of cell A electrode with aflat circled end made of gold with a diameter of 15 mm as anode were used The time of 15 minute and thepotential of 5kV were chosen as optimum time and potential for extraction For both substances calibrationcurve was obtained Linear ranges were 0 1 2 and 0 7 10 micro molar for octreotide and oxytocin respectively Detection limits were 0 02 and 0 08 micro molar for octreotide and oxytocin respectively Relative standard deviations of 2 5 for octreotide and 2 9 for oxytocin were obtained Also the recoverypercentage was for octreotide 40 44 and 40 46 for oxytocin Considering these represented results it isexpected that this peptide analysis method whit advantage such as simplicity fast response suitabledetection limit and linear dynamic rang be used for quantitative analysis of peptides in biologicale andpharmacology fields Keywords Peptide drug Liquid liquid electroextraction Octreotide Oxytocin

تقي خياميان

محمدسراجي، حسن قاضي عسگر