7373

6879

كارشناسي ارشد

اصلاح نباتات

اصفهان: دانشگاه صنعتي اصفهان، دانشكده كشاورزي

1391

ده، 48ص.: مصور، جدول، نمودار

ص.ع.به فارسي و انگليسي

آقافخرميرلوحي، محمد مهدي مجيدي

فاطمه اميني

21/9/91

محمدرضا سبز عليان، طالبي بداف

1396/09/21

كتابنامه

كشاورزي

مهندسي كشاورزي

ID6879

به فارسي و انگليسي: قابل رويت در نسخه ديجيتالي

49 Evaluation of Genetic Diversity in Second Generation Synthetic Progenies of Tall Fescue using EST SSR Mohsen Jafarzadeh Mohsenjafarzadeh35@yahoo com Department of Agronomy and Plant Breeding Isfahan University of Technology Isfahan 84156 83111 IranDegree M Sc Supervisor A F Mirlohi mirlohi@cc iut ac irAbstractTall fescue Festuca aurudinace Schrab is one of the most important forage speciesworldwide Development of synthetic varieties is the most common breeding approach in thisplant The first synthetic generation is obtained through multi parental random crossing Selecting parental genotypes is mostly based on morphological traits that are highlyinfluenced by environmental conditions Molecular markers are not affected by environmentand may help selecting diverse parental genotypes more precisely Present study wasconducted to assess the molecular diversity of two syn2 populations one derived fromparental genotypes with high molecular distance and the other from parents with highmorphological distance From each population sixty random genotypes were evaluated using23 EST SSR primers From the 23 tested primers 18 were selected based on polymorphicband development Totally 80 bands were produced in which 71 showed polymorphism Within population genetic similarity coefficient ranged from 0 51 to 0 95 for the syn2population having molecularly diverse parental genotypes This value ranged from 0 48 to0 96 for the syn2 population derived from morphologically diverse parents Results of clusteranalysis at similarity coefficient of 0 62 and 0 69 divided population 1 and 2 into 10 and 21groups respectively For both populations principle component analysis partially confirmedthe results obtained for cluster analysis Analysis of molecular variance was conductedbetween these two populations Based on this 74 17 percent of observed diversity was relatedto within population and just 25 83 percent was related to between population diversityrespectively The Euclidian distance between the two syn2 populations was 0 19 which washigher than what it was 0 13 for the syn1 populations Results showed that using molecularmarkers in parental selection it is possible to eliminate environmental effects that are evidentin morphological characteristics and the selected diversity in the parents is transmitted to thesubsequent generations Keywords tall fescue molecular markers synthetic varieties

آقافخرميرلوحي، محمد مهدي مجيدي

فاطمه اميني

محمدرضا سبز عليان، طالبي بداف