7376

6882

كارشناسي ارشد

اصلاح نباتات كشاورزي

اصفهان: دانشگاه صنعتي اصفهان، دانشكده كشاورزي

1391

دوازده، 57ص.: مصور، جدول، نمودار

ص.ع. به فارسي و انگليسي

فخرميرلوحي، محمدرضا سبزعليان

بهرام شريف نبي

21/9/91

محمدمهدي مجيدي، مسعود بهار

1396/09/21

كتابنامه

كشاورزي

مهندسي كشاورزي

ID6882

به فارسي و انگليسي: قابل رويت در نسخه ديجيتالي

28Detection of Insecticidal Alkaloid Genes in Neotyphodium Endophytes of Five Grass Species Maryam Zahediannzhad Ma zahediyan@gmail com Data of submission September 19 2012 Department of Agronomy and Plant Breeding Isfahan University of Technology Isfahan 84156 83111 Iran Degree M Sc Language Farsi 1 A F Mirlohi Ph D Professor Supervisor mirlohi@cc iut ac ir 2 M Sabzalian Assis Prof Supervisor sabzalian@cc iut ac ir Abstract Symbiotic relationship between some members of Poacea family and a group of fungi called endophytes has been established These symbionts belong to Clavicipitacae family previously known as simple parasites of plants These fungi grow intercellularly in the leaf sheets of host plants then enter the seed through the overy and stay in aleuron layer until seed germination Presence of these fungi in plants leads to resistance against drought improves photosynthesis and resistance to pests and diseases Several alkaloids such as lolin peramin lulitrom and ergovalin produced by the endophytes are responsible for the plant resistance against biotic and a biotic stresses Thus knowledge on the genes involved in the alkaloids biosynthesis is useful in the field of plant breeding Keeping these beneficial fungi in host crops may lead to persistant production during years and in different environments and will provide scientists with new sources of germplasm for selecting resistant plants This study was designed to detect genes of endophytic fungi associated with insecticidal alkaloids in several forage grasses including Melica persica Festuca arundinacea Lolium prenne and Bromus tomentellus After isolation morphological characteristics were investigated on PDA medium Then genomic DNA was extracted from isolates to confirm the genus identity using specific primers IS1 IS3 ITS1 ITS4 and 11 1 11 2 In most of the isolates the 444 bp band was amplified with the IS1 IS3 primer pair Also in a number of isolates the 1000 bp band was amplified by 11 1 11 2 primers No amplification was possible with ITS1 ITS4 primers Based on morphological characteristics and specific primers most endophytes isolated from Festuca arundinaceae Lolium prennea Melica persica and Bromus tomentellus were identified as Neotyphodium To detect genes involved in synthesis of alkaloids lolin peramin ergovalin and lolitrum PCR reactions were set using specific primers Amplification of bands with the expected size was taken as evidence for the presence of the genes in the isolates All the expected bands were amplified in an isolate LpMd that belonged to Lolium prennea This may indicate the presence of all four genes in this isolate On the other hand no amplification was observed for isolates Lp and MpDe belonging to the hosts Lolium prennea and Melica persica respectively Our result showed that designing specific primers it may be possible to detect genes responsible for insecticidal alkaloids in Neotyphodium isolated from different grass hosts Key Words endophytes fungi Symbiotic PCR Alkaloid gene

فخرميرلوحي، محمدرضا سبزعليان

بهرام شريف نبي

محمدمهدي مجيدي، مسعود بهار