7554

7026

كارشناسي ارشد

بوم شناسي ْبزيان شيلاتي

اصفهان: دانشگاه صنعتي اصفهان، دانشكده منابع طبيعي

1391

سيزده،61ص.: مصور،جدول،نمودار ﴿رنگي﴾

ص.ع.به فارسي و انگليسي

فاطمه پيكان حيرتي

نصراله محبوبي صوفياني، سالار درافشان

14/12/91

حسين مرادي، اميدوار فرهاديان

مهندسي منابع طبيعي

ID7026

به فارسي و انگليسي: قابل رويت در نسخه ديجيتالي

The Sublethal Effects of Cadmium on Some Hematological Parameters and Gill Histopathology in Sterlet Acipenser ruthenus Marziyeh Orojali Email m orojali@ymail com Date of Submission 16 12 2012 Department of Natural Resources Isfahan University of Technology Isfahan 84156 83111 Iran Degree M Sc Language Farsi Supervisor fheyrati@cc iut ac ir Abstract Cadmium is a heavy metal commonly introduced into the Caspian Sea and its rivers The aim of this study was to investigate the effects of sublethal concentrations of cadmium on some hematological parameters and gill histopathology in sterlet Asipenser ruthenus A total of 60 fish with average weight 41 69 0 95 g and the mean length 23 98 0 14 cm were divided into 4 groups with 3 replicates Fish were exposed at zero control 16 32 and 64 g L Cd concentration for 21 days in 95 L tanks under laboratory condition in semi static flow system Physicochemical factors of water were measured during the experiment including dissolved oxygen temperature pH EC nitrate phosphate alkalinity and hardness Blood samples were taken of caudal region of the fish using five percent EDTA as an anticoagulant and gill tissues were fixed in Bouin s solution for histopathological studies using hematoxylin eosin method Gill tissues showed several histological changes such as hyperplasia hypertrophy of epithelial cells and epithelial edema as well as necrosis rings and fusion of gill filaments and secondary lamellae There were a significant relationship between the intensity of histological changes of the gills and Cd concentration where the highest adverse effects on the gills were observed in fish treated with 64 g L of Cd in comparison with control Hematology parameters were been measured including red and white blood cell counting hematocrit hemoglobin and secondary indices such as mean corpuscular volume MCV mean corpuscular hemoglobin MCH and mean corpuscular hemoglobin concentration MCHC and the ratio of white to red cells As a whole results showed that only hematocrit and hemoglobin in groups treated with 32 and 64 g L of Cd increased significantly compared to control p 0 05 Other hematological factors didn t show any significant changes among groups p 0 05 In conclusion the results of this study showed that subletal concentration of cadmium can cause deleterious effects on gill as well as blood in sterlet Key words Sterlet Acipenser ruthenus Cadmium Sublethal Hematological parameters Histopathological changes Gill

فاطمه پيكان حيرتي

نصراله محبوبي صوفياني، سالار درافشان

حسين مرادي، اميدوار فرهاديان