پديد آورنده :
عربشاه، زهرا
عنوان :
تنوع مولكولي ويروس موزاييك يونجه و جمع آوري شده از ميزبان هاي مختلف در استان هاي اصفهان و چهارمحال وبختياري
مقطع تحصيلي :
كارشناسي ارشد
گرايش تحصيلي :
بيماري شناسي گياهي
محل تحصيل :
اصفهان: دانشگاه صنعتي اصفهان، دانشكده كشاورزي
صفحه شمار :
سيزده،78ص.: مصور،جدول،نمودار
يادداشت :
ص.ع.به فارسي و انگليسي
توصيفگر ها :
RT-PCR , ژن پروتيين پوششي , همرديف سازي , درخت تبارزايي
تاريخ نمايه سازي :
17/1/92
استاد داور :
مسعود بهار، احمد ارزاني
چكيده فارسي :
به فارسي و انگليسي: قابل رويت در نسخه ديجيتالي
چكيده انگليسي :
Molecular variation of alfalfa mosaic virus isolated from different hosts in Isfahan province Zahra Aarabshah zahraarabshah@yahoo com Date of submission January 21 2013 Department of Plant Protection Isfahan University of Technology Isfahan 84156 83111 Iran Degree M Sc Language Farsi 1 A Massah Ph D Assist Professor Supervisor amassah@cc iut ac ir Abstract Alfalfa mosaic virus AMV belongs to the genus Alfamovirus in the family Bromoviridae occurs worldwide The virus can naturally infect many herbaceous and some woody plant hosts 150 species in 22 families such as alfalfa potato bean pepper pea soybean basil and causes severe losses Alfalfa mosaic virus is transmitted by aphids pollen seed and dodder Since there are no available molecular data for AMV isolates in Iran During a survey in 2011 and 2012 to detect and determine certain molecular properties of AMV leaf samples showing viral symptoms were collected from alfalfa potato bean soybean pepper and basil crops in Isfahan and Charmahal va bakhtiari provinces Total RNA was extracted from leaf samples and subjected to RT PCR using specific primer pairs of AMV AlMV 3pr AlMV 5pr AMV F2 AMV R2 Several samples amplified expected sizes of 901 bp and 669 bp in RT PCR respectively The amplicons of 13 isolates including ADA4 Daran alfalfa AK2 Karkhane ghand alfalfa SMZ Daran potato PSH Shahrkord potato 13LM Shahrkord bean PPF Daran pepper PPB4 Dorcheh pepper CLD Dorcheh clover LPK Kelishad tomato PKH Khomeini shahr pepper TBN Daran WD Dastgerd chenopodium and WS Dastgerd weed representing different hosts and regions were cloned and sequenced The BLAST search revealed high homology between the Iranian isolates and other AMV isolates available at GenBank Multiple alignment of coat protein amino acid sequence of Iranian isolates and those of AMV isolates available at GenBank revealed homology ranging from 97 7 100 between Iranian isolates Nine isolates showed 100 homology and Shahrkord bean isolate showed the lowest homology with other isolates All Iranian isolates showed highest homology ranging from 98 6 99 5 to American soybean isolate with accession number HQ185569 and China alfalfa isolate with accession number HQ316647 whereas they showed lowest homology ranging from 95 2 97 2 to Korean potato isolate with accession number AB126032 and New Zealand alfalfa isolate with accession number U12510 In phylogenetic analysis Iranian isolates were placed in one cluster and there is no separation based on hosts or geographical regions For comparison of biological properties of isolates potato and alfalfa isolates were mechanically inoculated on test plants including Chenopodium quinoa Gomphrena globosa Nicotiana rustica Nicotiana glutinosa Cucumis sativus Vigna unguiculata and Solanum tuberosum There are no differences in symptoms of test plants between Daran potato and Dastgerd alfalfa isolates The infection of test plants to AMV was confirmed via RT PCR Key word Alfalfa mosaic virus RT PCR Coat protein gene Alignment Phylogenetic tree
استاد داور :
مسعود بهار، احمد ارزاني
