پديد آورنده :
طلايي، نسيم
عنوان :
استفاده از لايه آلرون بذر جو به عنوان سيستم مدل جهت بررسي اثر ساليسيليك اسيد بر الگوي ترشح و فعاليت پروتئيني
مقطع تحصيلي :
كارشناسي ارشد
گرايش تحصيلي :
بيوتكنولوژي
محل تحصيل :
اصفهان: دانشگاه صنعتي اصفهان، دانشكده كشاورزي
صفحه شمار :
چهارده،67ص.: مصور،جدول،نمودار
يادداشت :
ص.ع.به فارسي و انگليسي
استاد راهنما :
آذر شاه پيري، مهدي شمس آرا
توصيفگر ها :
آلفا آميلاز , ليميت دكستريناز , آسكوربات پر اكسيداز
تاريخ نمايه سازي :
4/2/92
استاد داور :
آقا فخر ميرلوحي، مهديه غلامي
چكيده فارسي :
به فارسي و انگيسي: قابل رويت در نسخه ديجيتالي
چكيده انگليسي :
Aleurone layer of barley seed as model system for analysis of the effect of salicylic acid on protein secretion and activity profiles Nasim Talaei Nasim talaei@yahoo com Date of Submission January 15 2013 Department of Agricultural Biotechonolgy Isfahan University of Technology Isfahan 84156 83111 Iran Degree M Sc Language Farsi 1 A Shahpiri Ph D Assist Professor Supervisor a shahpiri@cc iut ac ir 2 M Shamsara Ph D Assist Professor Supervisor shamsara@nigeb ac ir Abstract Cereal seed germination involves mobilization of the storage reserves in the starchy endosperm necessary to support seedling growth The aleurone layer plays a key role in germination by responding to hormone signals from embryo and producing hydrolases that are secreted into the starchy endosperm for degradation of storage products In the present work the spatio temporal patterns of 4 amylase and limit dextrinase LD were monitored in dissected aleurone layers from barley Hordeum vulgare seeds incubated for different times 6 72 h in the presence of the hormones gibberellic acid GA abscisic acid ABA and salicylic acid SA The release of the proteins from the aleurone layer to the culture supernatant was also monitored by immunoblotting and the timing of the release of LD was found to differ from that of 4 amylase and suggested to depend on the programmed cell death PCD The release 4 amylase from aleurone layer to culture supernatant however was observed before PCD The amount of total protein considerably decresead in aleurone layer treated with 1 mM SA while the amount of total protein in aleurone layer treated with 20 M SA was even more than aleurone layer incubated in control medium which could be due to the stopping of protein secretion from aleurone layer in response SA and accumulation of proteins in aleurone layer The aleurone layers were also treated with a combination of hormones GA ABA and GA SA and the appearance of 4 amylase and LD was investigated in both alurone layer and supernatant The resutls show that even though the low amount of these two enzymes are present in aleurone layer treated with either ABA or SA these enzyme can be secreted to supernatant only in the presence of GA In addition in this study the activity of ascorbate peroxidase APX as one of the enzyme involved in the ascorbate glutathione cycle was analyzed in soluble proteins from aleurone layer The results show that the activity of APX is considerably decreased in aleurone layer teated with GA after 48 h whereas these changes was not observed in aleurone layer treated with either ABA or SA Keywords aleurone layer 4 amylase limit dextrinase salicylic acid ascorbate peroxidas
استاد راهنما :
آذر شاه پيري، مهدي شمس آرا
استاد داور :
آقا فخر ميرلوحي، مهديه غلامي