پديد آورنده :
فيضي گيلانده، ندا
عنوان :
استخراج داروي مگزيلتين از پلاسما و ادرار به وسيله ي تكنيك ريز استخراج فاز مايع با فيبر توخالي، مشتق سازي پيش ستون و اندازه گيري توسط كروموتوگرافي مايع با آشكار ساز فلورسانس
مقطع تحصيلي :
كارشناسي ارشد
محل تحصيل :
اصفهان: دانشگاه صنعتي اصفهان، دانشكده شيمي
صفحه شمار :
سيزده،60ص.: مصور،جدول،نمودار
يادداشت :
ص.ع.به فارسي و انگليسي
استاد راهنما :
محمد سراجي
توصيفگر ها :
ريز استخراج مايع-مايع-مايع , ريز استخراج بر اساس فيبر توخالي با فاز پذيرنده سولفوريك اسيد , مشتق ساز اورتوفتالدهايد , دستگاه كروماتوگرافي مايع با كارايي بالا و آشكار ساز فلورسانس
تاريخ نمايه سازي :
26/2/92
استاد داور :
تقي خياميان، محمدتقي جعفري
چكيده فارسي :
به فارسي و انگليسي: قابل رويت در نسخه ديجيتالي
چكيده انگليسي :
60 Extraction of mexiletine from plasma and urine using liquid phase microextraxtion followed by pre column derivatization high performance liquid chromatography fluorescence detection Neda Feizi Gilandeh Department of Chemistry Isfahan University of Technology Isfahan 84156 83111 Iran Degree M Sc Lanquage Farsi Superviser Mohammad Saraji Email saraji@cc iut ac ir Abstract Mexiletine hydrochloride is an orally active class I antiarrythmic agent A sensitive and specific high performance liquid chromatography HPLC method has been developed for the determination and quantification of mexiletine in human urine and plasma hollow fiber liquid liquid liquid microextraction HF LLLME followed by a reverse phase HPLC with a pre column derivatization with ortho phthalaldehyde OPA and fluorescence detectionfor analysis of MEX in human urine and plasma 1 2 Chromatographic separation was performed on a C ODS column shim pack 50mm 4mm i d with 3 m particle size with mobile phase consisting of acetonitrile and water 80 20 v v isocrtic and the flow rate was set at 1ml min followed by fluorescence detection ex 350nm and em 445nm All variables effecting the exraction of analyte including acceptor organic solvent type concentration of NaOH in donor phase ionic strength of the sample and exraction time were studied The assay was linear in the concentration range 1 200 g L 1 r2C0 996 The relative standard deviation for urine was lower than 0 8 and for plasma was lower than 1 6 for both urine and plasma The enrichment factor was 153 and the detection limit is 0 3ppb the method was successfully applied for the analysis of MEX in urine and plasma samples Keywords HF LLLME Derivatization OPA HPLC FLD mexiletine Plasma and Urine
استاد راهنما :
محمد سراجي
استاد داور :
تقي خياميان، محمدتقي جعفري
