پديد آورنده :
ميرمقتدايي، ليلا
عنوان :
طراحي، ساخت و ارزيابي يك نانوحسگر و يك زيست حسگر به منظور اندازه گيري اسيد فوليك در آرد گندم
گرايش تحصيلي :
علوم و صنايع غذايي
محل تحصيل :
اصفهان: دانشگاه صنعتي اصفهان، دانشكده كشاورزي
صفحه شمار :
سيزده،125ص.: مصور،جدول،نمودار﴿رنگي﴾
يادداشت :
ص.ع.به فارسي و انگليسي
استاد راهنما :
مهدي كديور، محمد شاهدي
استاد مشاور :
علي اصغر انصافي
توصيفگر ها :
الكتروشيميايي , نانو ذرات طلا , داكسي ريبونو كلئيك اسيد , حساسيت , انتخاب گري
تاريخ نمايه سازي :
23/2/92
استاد داور :
مرتضي صفري، مهران ميراوليايي، جواد كرامت
كد ايرانداك :
ID506 دكتري
چكيده فارسي :
به فارسي و انگليسي: قابل رويت در نسخه ديجيتالي
چكيده انگليسي :
Design fabrication and evaluation of a nanosensor and a biosensor for measuring of folic acid in wheat meal Leila Mirmoghtadaie leila m@ag iut ac ir Date of Submission 2013 1 21 Department of Food Science and Technology College of Agriculture Isfahan University of Technology Isfahan 84156 83111 Iran 1st Supervisor Mahdi Kadivar kadivar@cc iut ac ir 2nd Supervisor Mohammad Shahedi shahedim@cc iut ac ir Advisor Ali Asghar Ensafi ensafi@cc iut ac ir Department Graduate Program coordinator Mohammad Mahdi Majidi Department of Food Science and Technology College of Agriculture Isfahan University of Technology Isfahan 84156 Iran Department of chemistry Isfahan University of Technology Isfahan 84156 IranAbstractThis study is consisting of two sections At first section a novel gold nanoparticle goldelectrode was used to folic acid determination In this part the selective oxidation of folicacid was investigated on the gold electrode After sensor optimization it was used to measurefolic acid by differential pulse voltammetry A good linear correlation was found between thepeak current of folic acid and its concentration in the range of 1 0 10 8 to 1 0 10 6 mol L 1with a detection limit of 7 50 10 9 mol L 1 In second section an electrochemical DNAbiosensor was proposed as a screening device for the rapid analysis of folic acid using apencil graphite electrode modified with salmon sperm ds DNA Solution pH DNAconcentration time of DNA deposition and potential of deposition was optimized with RSM After that the binding of folic acid to DNA immobilized on a pencil graphite electrode wasmeasured through the variation of the electrochemical signal of adenine Folic acid could bemeasured in the range of 0 1 10 0 mol L 1 with a detection limit of 1 06 10 8 mol L 1 Key Words Folic acid electrochemical Sensor Gold nanoparticle DNA IntroductionFolate includes folic acid and derivatives that occur in nature and that are required in thesingle carbon metabolism such as amino acid and nucleic acid biosynthesis as well as in celldivision and growth 7 Folic acid is commonly measured using such methods as HPLC MS spectroscopy ELISA and microbial methods However most of these methods are cost intensive and time consuming due to their long extraction and purification process times These shortcomings warrant the development of a new rapid sensitive and selective methodfor folic acid determination The principle of electrochemical sensors is based on the fact the current generated by theoxidation or reduction of an electroactive analyte on the working electrode surface ismeasured by the electrochemical detector within a specified fixed or varying potential 9 The combination of nanoparticles and electrochemistry lead to more reliable and powerfulmethods 8 Gold nanoparticle modified electrodes can be prepared using such differentmethods as gold colloid deposition and electrodeposition Amongst the different preparationmethods electrochemical deposition is a fast easy and convenient method for thepreparation of gold nanoparticles 2
استاد راهنما :
مهدي كديور، محمد شاهدي
استاد مشاور :
علي اصغر انصافي
استاد داور :
مرتضي صفري، مهران ميراوليايي، جواد كرامت
