پديد آورنده :
نقدي، ميترا
عنوان :
بهينه سازي فرايند استخراج بيوپليمركيتوزان از توده زيستي قارچ هاي زيگومايست
مقطع تحصيلي :
كارشناسي ارشد
گرايش تحصيلي :
مهندسي شيمي
محل تحصيل :
اصفهان: دانشگاه صنعتي اصفهان، دانشكده مهندسي شيمي
صفحه شمار :
دوازده،54ص.: مصور،جدول،نمودار
يادداشت :
ص.ع.به فارسي و انگليسي
استاد راهنما :
اكرم زماني، كيخسرو كريمي
توصيفگر ها :
فسفات آزادشده , رايزوپوس اورايزه , ديواره سلولي , اسيد سولفوريك و اسيد لاكتيك
تاريخ نمايه سازي :
24/4/92
استاد داور :
طيبه بهزاد، فاطمه بنا كاشاني
چكيده فارسي :
به فارسي و انگليسي: قابل رويت در نسخه ديجيتالي
چكيده انگليسي :
55 Optimization of Chitosan Extraction from Zygomycetes Fungal Biomass Mitra Naghdi M naghdi66@yahoo com 22 January 2013 Department of Chemical Engineering Isfahan University of Technology Isfahan 84156 83111 Iran Degree M Sc Language Farsi Supervisors Akram Zamani zamani akram@cc iut ac ir Keikhosro Karimi karimi@cc iut ac ir Abstract Chitosan a useful cationic polymer of glucosamine monomers is industrially produced via chemical deacetylation of extracted chitin from shellfish Recently cell wall of zygomycetes fungi has been introduced as an alternative source for chitosan production in which chitosan is produced via enzymatic deacetylation of chitin In this study a new method for optimum extraction of chitosan from fungal cell wall was suggested and the effects of sulfuric hydrochloric nitric acetic and lactic acids on the phosphate removal from the cell wall and also preservation of chitin and chitosan were investigated The efficient extraction method was involved employing sulfuric acid as the best acid for phosphate removal and treating with lactic acid for dissolution and extraction of chitosan Results showed that solutions of sulfuric acid caused chitosan to be insoluble in the acidic solutions at ambient temperature however alkali treatment recovered its solubility The yield of cell wall was measured to be 0 16 grams per gram of dried biomass Finally chitosan was extracted from the cell wall remaining materials by lactic acid treatment at ambient temperature Using the proposed method the yield of chitosan for the cell wall of Rhizopus oryzae was measured to be 12 3 at ambient temperature It is noteworthy that the new method was performed for commercial chitosan and the yield 86 9 was obtained The viscosity of commercial chitosan was measured before and after the process and the results showed that the viscosity remains unchanged during acid and alkaline treatments Beside chitosan the phosphate protein glucosamine and n acetyl glucosamine content of the fungal cell wall and commercial chitosan were also investigated and the results showed that the impurities of Rhizopus oryzae and commercial chitosan after sulfuric acid treatment were reduced to 1 and 0 7 respectively Furthermore the protein content of cell wall after alkali treatment was measured to be less than 5 of the dried biomass The morphology of cell walls subjected to treatment with 5 different acids was monitored by scanning electron microscopy SEM Keywords Fungal chitosan Released phosphate Rhizopus oryzae Cell wall Sulfuric acid Lactic acid PDF created with pdfFactory trial version www pdffactory com
استاد راهنما :
اكرم زماني، كيخسرو كريمي
استاد داور :
طيبه بهزاد، فاطمه بنا كاشاني
