پديد آورنده :
مسائلي، الهه
عنوان :
طراحي داربست هاي نانو الياف پلي هيدروكسي آلكانوات جهت مهندسي بافت عصب
محل تحصيل :
اصفهان: دانشگاه صنعتي اصفهان، دانشكده نساجي
صفحه شمار :
سيزده،159ص.: مصور،جدول،نمودار
يادداشت :
ص.ع.به فارسي و انگليسي
استاد راهنما :
محمد مرشد، محمد حسين نصر اصفهاني
توصيفگر ها :
سلول هاي شوان
تاريخ نمايه سازي :
26/6/92
استاد داور :
شادپور ملك پور، شهناز رضويي، صديقه برهاني
كد ايرانداك :
ID530 دكتري
چكيده فارسي :
به فارسي و انگليسي: قابل رويت در نسخه ديجيتالي
چكيده انگليسي :
Design of Nanofibrous Scaffolds of Poly Hydroxyalkanoates PHAs for Nerve Tissue Engineering Elahe Masaeli elahe mas@tx iut ac ir2 2T T Department of Textile Engineering Isfahan university of Technology Isfahan 84156 83111 Iran Mohammad MorshedP I morshed@cc iut ac ir 0F P Mohammad Hossein Nasr EsfahaniP II mh nasr@med mui ac ir 1F P Abstract Scaffolds that mimic physico chemical properties of extracellular matrix ECM are valuable to stimulate enhanced cell material interactions for tissue engineering applications Poly hydroxyl alkanoates PHAs are versatile natural polyesters which are attractive materials for tissue engineering due to their natural origin good mechanical properties and enhanced biocompatibility In this study we compared interactions of Poly 3 hydroxy butyrate PHB scaffolds fabricated by electrospinning and salt leaching procedures with monkey epithelial kidney cells Vero and mouse mesenchymal stem cells mMSCs Our results showed nanofibrous scaffolds can be a better substrate for cell attachment and morphology As Schwann cells SCs physiologically assist in directing the growth of regenerating axons we also assessed the potential of PHB Poly 3 hydroxybutyrate co 3 hydroxyvalerate PHBV nanofibrous scaffolds for nerve regeneration using SCs Our experiments indicated that the melting temperatures and crystallinity of PHB PHBV blends decreased as the PHBV weight ratio increased Digital image processing analysis also proved SCs orientation along the fiber direction of aligned mats To improve scaffolds biocompatibility we also functionalized scaffolds with ECM derived peptides to mimic naturally occurring ECM motifs for nerve regeneration Briefly proliferation and neural gene expressions of SCs cultured on bio functionalized scaffolds increased significantly which suggest that these substrates could serve as a potential scaffold for SCs differentiation and axon myelination Keywords Poly hydroxyalkanoates Scaffold Schwann cells Nerve tissue engineering I Department of Textile Engineering Isfahan University of Technology Isfahan 84156 83111 Iran II Department of Cell and Molecular Biology Cell Science Research Center Royan Institute for Animal Biotechnology ACECR Isfahan 81589 68433 Iran PDF created with pdfFactory trial version www pdffactory com
استاد راهنما :
محمد مرشد، محمد حسين نصر اصفهاني
استاد داور :
شادپور ملك پور، شهناز رضويي، صديقه برهاني
