7965

7400

كارشناسي ارشد

اصلاح نباتات

اصفهان: دانشگاه صنعتي اصفهان، دانشكده كشاورزي

1391

سيزده،65ص.: مصور،جدول،نمودار

ص.ع.به فارسي و انگليسي

آقافخرميرلوحي، خليل زينعلي نژاد

محمد مهدي مجيدي

2/7/92

احمد ارزاني، مهديه غلامي

مهندسي كشاورزي

ID7400

به فارسي و انگليسي: قابل رويت در نسخه ديجيتالي

Assessing cross pollination in sainfoin Onobrychis vicifolia Scop using ISSR markers Mostafa Arzhang mostafa arjang@yahoo com Date of Submission 15 january 2013 Department of Agronomy and Plant Breeding Isfahan University of Technology Isfahan 84156 83111 Iran Degree M Sc Language Farsi Supervisor 1 Mirlohi Ph D Professor Supervisor mirlohi@cc iut ac ir 2 Zaynali nezhad Ph D Supervisor khalil1381@yahoo com Abstract Onobrochys French grass is one of the most important foliage species in providing and compensation of foliage shortage in Iran In most of scientific literatures onobrochys vicifolia has been introduced as out pollination but its mechanism and ways are not clear Knowledge of pollination system is so efficient and necessary to improve breeding program for onobrochys vicifolia For this present research was conducted to determine out pollination of onobrochys vicifolia in natural and controlled condition using marker ISSR Based on initial experiment plan the number of four genotypes were cloned to determine pollination rate At first their polymorphism was evaluated using marker ISSR The clones were planted in May 2011 in three distinct regions as factorial experiment based on completely randomized block designs in two replicates In each region three genotypes having two replicates for open pollination and the same three genotypes with two replicates were planted for controlled pollination Results for variance analysis for traits related to seed yield and artificial tripping in onobrochys vicifolia showed that in contrast to previous studies which stated onobrochys out pollination between zero and 37 onobrochys out pollination was estimated as 100 percent which has great deal of importance in experiment goal based breeding program Evaluation of traits related to plant reproductive stage traits of panicle number and flower in panicle under controlled and pollinated conditions indicated that plant reproductive stage were not affected by environment rather plant vegetative growth during growing season is influenced by plant pollination conditions Evaluation of molecular data of marker ISSR confirmed results of variance analysis of traits related to seed yield Grouping S1 generation and parents in each region indicated that S1 distributed uniformly in whole dendrogram It has been suggested that variation present in parents was distributed uniformly in S1 pollination Results of principle component analysis generally confirmed traits classification and variance analysis results related to seed yield Keywords out pollination S1 generation Onobrochys vicifolia ISSR PDF created with pdfFactory trial version www pdffactory com

آقافخرميرلوحي، خليل زينعلي نژاد

محمد مهدي مجيدي

احمد ارزاني، مهديه غلامي