8108

565 دكتري

دكتري

شيمي تجزيه

اصفهان: دانشگاه صنعتي اصفهان، دانشكده شيمي

1392

بيست و چهار،186ص.: مصور،جدول،نمودار

ص.ع.به فارسي و انگليسي

علي اصغر انصافي، بهزاد رضايي

13/8/92

مرتضي طالبي، تقي خياميان، محمدتقي جعفري

شيمي

ID565 دكتري

به فارسي و انگليسي: قابل رويت در نسخه ديجيتالي

190 Design and construction of electrochemical DNA nano biosensors for determination of Sudan II in food Amitrole as a herbicide and distinguish of DNA damage Maryam Amini E mail Maryam Amini@yahoo com Department of Chemistry Isfahan University of Technology Isfahan Iran Supervisors Professor Ali A Ensafi and Professor Behzad Rezaei E mail address Ensafi@cc iut ac ir rezaei@cc iut ac ir Department Graduate Program Coordinator Hossein Tavakol Department of Chemistry Isfahan University of Technology Isfahan Iran Tel Fax Abstract In the first part of this thesis the interaction of ds DNA and sudan II dye was inspected For this purpose pencil graphite electrode PGE and differential pulse voltammetry DPV technique were used Using adsorptive stripping voltammetry ds DNA in acetate buffer solution was immobilized on the surface of the electrode Furthermore using DPV method guanine and adenine signals after interaction of ds DNA and sudan II were achieved Under the optimum condition with following the guanine and adenine signals a linear dynamic range LDR between 0 5 6 0 g mL 1 was achieved The limit of detection LOD based on guanine and adenine signals were 0 43 and 0 48 g mL 1 respectively In order to investigate the mechanism of interaction DPV and UV vis methods were used These investigations showed that the type of interaction is intercalation Finally the effect of interference species was investigated and the amount of sudan II in chili and ketchup souses were determined In the second section of this thesis the amount of sudan II was determined using DPV method and PGE Using this method LDR between 0 0015 3 0000 g mL 1 and LOD 0 70 ng mL 1 were achieved In second stage of this investigation interaction between amitrole and ds DNA using differential pulse voltammetry DPV technique were used Under the optimum condition LDR between 0 025 2 400 ng mL 1 and LOD 0 017 ng mL 1 were achieved In the third section ds DNA damage due to Cr VI GSH H2O2 was investigated In order to optimize the parameters and ds DNA damage inspections DPV method and PGE were used In order to follow the ds DNA damage methylene blue as an electroactive probe was used To investigate the amount of ds DNA damage the ratio of MB signal before and after damage was inspected Finally the time constant of ds DNA damage was calculated In the fourth section ds DNA damage due to catechol in presence of metal ions was investigated In order to investigate the ds DNA damage electrochemical impedancePDF created with pdfFactory trial version www pdffactory com

علي اصغر انصافي، بهزاد رضايي

مرتضي طالبي، تقي خياميان، محمدتقي جعفري