پديد آورنده :
حيدري بفروئي، اسماعيل
عنوان :
تهيه زيست حسگرهاي جديد برپايه DNA جهت آناليز تركيبات مهم دارويي و بكار گيري نانو ساختارها به منظور بررسي تخريب DNA و مطالعه رهش دارو از نانو ذرات درماني
مقطع تحصيلي :
كارشناسي ارشد
گرايش تحصيلي :
شيمي تجزيه
محل تحصيل :
اصفهان: دانشگاه صنعتي اصفهان، دانشكده شيمي
صفحه شمار :
بيست، 169ص.: مصور،جدول،نمودار
يادداشت :
ص.ع.به فارسي و انگليسي
استاد راهنما :
علي اصغر انصافي
استاد مشاور :
بهزاد رضايي
تاريخ نمايه سازي :
13/8/92
استاد داور :
مرتضي طالبي، حسن قاضي عسگر، محمد سراجي
كد ايرانداك :
ID566 دكتري
چكيده فارسي :
به فارسي و انگليسي: قابل رويت در نسخه ديجيتالي
چكيده انگليسي :
Preparation of new DNA biosensors for analysis of some important pharmaceutical compounds and application of nanostructures for investigation of DNA damage and drug release profile from therapeutic nanoparticles Esmaeil Heydari Bafrooei E mail Heydari @gmail com September Department of Chemistry Isfahan University of Technology Isfahan Iran Supervisor Professor Ali Asghar Ensafi E mail address ensafi@cc iut ac ir Advisor Professor Behzad Rezaei Department Graduate Program Coordinator Dr Hossein Tavakol Department of Chemistry Isfahan University of Technology Isfahan Iran Tel Fax Abstract Multi wall carbon nanotubes MWCNTs and titanume oxide nano particles TiO2NPs are widely used in the fabrication of immunosensors because of their high electrical conductivity high chemical stability and extremely high mechanical strength DNA is an important biological polymer which is classi ed as a natural negatively charged polyelectrolyte due to its phosphate groups It can be immobilized onto carbon nanotubes via covalent and noncovalent interactions In this study firstly using a positively charged polyelectrolyte PDDA as dispersant of MWCNTs a method for immobilization of DNA on the MWCNTs and pencil graphite electrode PGE was developed The MWCNTs not only display unique electron transfer properties that induce the conductivity of PDDA and improve electron transfer characteristics they also increase the amount of positively charged polyelectrolyte deposited on the electrode Then using constructed DNA biosensor and different electrochemical methods cyclic voltammetry differential pulse voltammetry electrochemical impedance etc the interaction of several drugs with DNA was studied The decrease in intensity of the guanine and adenine oxidation signals and the change in intensity and position of the oxidation signals of riboflavin codeine and morphine was used as an indicator for the sensitive determination of these drugs Under optimized conditions the detection limits of the above compounds are and g mL respectively The biosensor was applied to validate its capability for the analysis of riboflavin codeine and morphine in real samples In continuing an electrocatalytic voltammetric method to assess total antioxidant capacity TAC using DNA modi ed PGE was developed studied The biosensor developed was applied to the determination of TAC in several beverages and the results were compared with those attained using other methodologies to obtain an overall picture of the antioxidant pro le Then A DNA based biosensor was fabricated for the first time for the electrochemical study of transition metal catalyzed oxidation of sulfite At the final section a simple electrochemical method was used to directly assess the drug release profile from therapeutic nanoparticles The method is based on the multiple pulse amperometric MPA measurement of the oxidation and reduction of doxorubicin released from liposome at a multiwalled carbon nanotube modified glassy carbon electrode MWCNT GCE Keywords DNA based biosensor DNA damage Drug relese Antioxidant capacity Electrochemical impedance spectroscopy Voltammetry PDF created with pdfFactory trial version www pdffactory com
استاد راهنما :
علي اصغر انصافي
استاد مشاور :
بهزاد رضايي
استاد داور :
مرتضي طالبي، حسن قاضي عسگر، محمد سراجي
