بررسي تنوع ژنتيكي تمشك خزري ﴿.Rubus hyrcanus Juz ﴾ با استفاده از نشانگرهاي ISSR و مورفولوژيك در ايران

چكيده انگليسي :

71 Genetic diversity of Caspian blackberries Rubus hyrcanus in Iran using ISSR and morphological markers Ehsan sedighi marnani esis999@yahoo com Submitted on 2013 Department of Agronomy and Plant Breeding Isfahan University of Technology Isfahan 84156 83111 Iran Degree M ScLanguage FarsiM Rahimmalek Assist Prof supervisor mrahimmalek@cc iut ac irAbstract Rubus is a member of the Rosaceae family This plant has more than 750 plantspecies in the world which are divided into two groups including blackberries and redraspberry This plant has a wide range of ploidy levels from 2X to 14X and the basicchromosome number of Rubus is seven This fruit has unique properties and is rich inantioxidants vitamins A B and C The roots and leaves have medicinal properties Inaddition the boiled leaves are consumable There are seven species of Rubus in Iran thatall belong to blackberries Caspian raspberry R hyrcanus is a species of blackberrywhich is scattered around the Caspian Sea and there are populations of this species inIran Information about variation within a species provides new approaches aboutevolutionary and conservation genetic strategies of this species Morphological andISSR molecular markers were used to study the genetic relationships of 25 populationssampled from three populations that geographically well distributed around the CaspianSea The morphological traits were measured and recorded based on the specified codes In addition R discolor was also included as control sample to ensure the validity ofcollection In this study 20 primers were used in which 10 of them had highpolymorphism 10 primers produced 204 bands 157 of which were polymorphic Cluster analysis revealed two main groups The first group includes samples from theNorth West NW and North East NE while group 2 included the Central C one R discolor separated from all R hyrcanus in dendrogram The cluster analysis was alsoperformed based on morphological traits Morphological and Principle CoordinateAnalysis PCoA confirmed the molecular cluster analysis in most cases The samplescollected from central regions mostly located in sandy soils near the coastal areas ofCaspian Sea while the NE and NW populations were collected from forests in themountain with high organic matter in the soil NW and NE populations had relativelysimilar altitudes while the Central one was located in lower altitude Among studiedsamples C population showed higher diversity 0 283 0 208 in comparison withothers Finally narrow genetic base and low diversity was obtained for R hyrcanus According to results of this study the probable origin of this species is central regionsof Caspian Sea during the period of time the seeds of berries were scattered in NE andNW regions The Alborz Mountains prevented seed dispersal and development of thisspecies into other regions of the country It is only as a hypothesis and further studiescan lead to more insightful results Keywords Fennel blackberry Rubus hyrcanus ISSR Genetic variation morphology

صديقي، احسان

بررسي تنوع ژنتيكي تمشك خزري ﴿.Rubus hyrcanus Juz ﴾ با استفاده از نشانگرهاي ISSR و مورفولوژيك در ايران

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