بررسي تجربي، بهينه سازي شرايط عملياتي و مدل سازي رياضي استخراج فوق بحراني تركيب كوئرستين از گلبرگ هاي گياه گل محمدي

چكيده انگليسي :

Experimental Investigation and Optimization of Supercritical Extraction of Quercetin from Rosa damascena Mill using Design of Experiments and Mathematical Modeling Seyed Omid Mousavi o mousavi@ce iut ac ir Date of submission 14 September 2013 Department of Chemical Engineering Isfahan University of Technology Isfahan 84156 83111 Iran Degree M Sc Language Farsi Supervisor Prof Seyyed Mohammad Ghoreishi ghoreshi@cc iut ac ir Abstract Rosa damascene which grows in most parts of Iran is a source of Flavonoid and antioxidant compounds like Quercetin and Kaempferol which are widely used in medical and sanitary industries Quercetin is a polar polyphenolic compound which is used in illness treatment like Artery stenosis Cataracts Dypertension Diabetes and Colon Cancer The common technique to extract plant ingredients is the conventional method such as Soxhlet with organic solvents such as ethanol and methanol But for pharmaceutical and medicinal applictions a suitable method for extraction and purification without utilization of toxic organic solvents is needed Supercritical fluid extraction especially supercritical carbon dioxide extraction as an efficient extraction method has attracted attention during the last decades as its advantages include being non explosive non toxic and available in high purity with low cost non solvent residues In this study organic solvent extraction method and supercritical carbon dioxide extraction of Rosa damascena was compared The extraction of Quercetin was investigated by modified supercritical CO2 with constant volume of co solvent 1 ml 20 min of static time and 0 674 mm of average particle size Design of experiment carried out with response surface methodology RSM using Minitab software Supercritical CO2 extraction was carried out under the following operating conditions 35 55 C 10 30 MPa 0 3 1 5 ml min CO2 flow rate 40 120 min extraction dynamic time Response surface analysis verified that the data were adequately fitted to second order polynomial model and the statistical analysis also showed that the linear and quadratic terms of pressure temperature CO2 flow rate and extraction dynamic time as well as the interactions of pressure and temperature and temperature and flow rate have considerable effects on the proposed model of Quercetin recovery based on coded variables R2 and modified R2 of the model are 92 99 and 86 86 respectively It was predicted that the optimum extraction conditions within the experimental ranges would be the extraction pressure of 25 55 MPa and temperature of 45 7 oC with flow rate of 0 81 ml min and extraction time of 108 68 min Under such conditions the recovery of Quercetin was predicted to be 31 75 Moreover in this work a mathematical modeling for Quercetin extraction from Rosa damascena was performed by modified supercritical carbon dioxide based on the differential mass balance Density and viscosity of dense gases mixture was obtained by Peng Robinsson PR equation of state with the van der Waals mixing rules and Chung et al respectively Mathematical model parameters are effective pore diffusivity film mass transfer coefficient axial dispersion and distribution coefficient The first three parameters were obtained from empirical equations and the distribution coefficient between solid and solvent has been determined by thermodynamic modeling of solubilities Finally operating conditions were optimized by means of Genetics Algorithm method in Matlab By this method optimal operating conditions were observed at 42 56 C 25 62 MPa 1 158 ml min and 101 66 min dynamic time to achieve 31 72 recovery The experiments from RSM corresponded to GA results well Keywords Rosa damascena Quercetin Supercritical CO2 extraction Response surface methodology Genetic algorithm Mathematical modeling PDF created with pdfFactory trial version www pdffactory com

موسوي، اميد

بررسي تجربي، بهينه سازي شرايط عملياتي و مدل سازي رياضي استخراج فوق بحراني تركيب كوئرستين از گلبرگ هاي گياه گل محمدي

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