8751

8112

كارشناسي ارشد

شيمي تجزيه

اصفهان: دانشگاه صنعتي اصفهان، دانشكده شيمي

1392

سيزده،70ص.: مصور،جدول،نمودار(رنگي)

ص.ع.به فارسي و انگليسي

محمدتقي جعفري، محمد سراجي

31/1/93

تقي خياميان، حسن قاضي عسگر

شيمي

ID8112

70 Design and construction of an intermediate connecting headspace solid phase microextraction with electrospray ionmobility spectrometry and use it to determination of venlafaxine in urine and plasma samples Amir Hossin Ameri amirameri511@yahoo com January 13 2014 Department of Chemistry Isfahan University of Technology Isfahan 84156 83111 IranDegree M Sc Lanquage FarsiSuperviser Mohammad Taghi Jafari Email jafari@cc iut ac irMohammad Saraji Email saraji@cc iut ac irAbstract In this research headspace solid phase microextraction technique coupled with electrospray ionizationion mobility spectrometry ESI IMS for the first time The proposed method has been applied for theextraction and determination of venlafaxine in the biological samples such as urine and plasma A desorptionchamber as interface was constructed for online connection of SPME technique to ESI IMS Analyte wasdesorbed by electrospray solvent and entered into the electrospray needle The solid phase microextraction SPME fiber coated with polypyrrole sol gel composite was prepared using a three electrodeelectrochemical system and directly deposited on a stainless steel wire by applying a constant potential 1 2V for 1000 s The effect of different parameters including extraction time temperature pH and ionicstrength were investigated and optimized Under the optimized conditions calibration curves were plottedindicating the linear dynamic range of 0 5 50 1 85 and 20 1000 g L for standard water spiked urine andspiked plasma samples respectively The relative standard deviation for the drug was less than 11 and thedetection limits 0 2 0 6 11 g L was obtained for the drug extracted from water spiked urine and spikedplasma respectively The relative recoveries obtained were 80 and 93 for spiked urine and plasmasamples respectively Finally some real samples of urine and plasma were analyzed and the obtained resultsreveal the capability of the proposed method for venlafaxine analysis in biological matrices Key Words Solid phase microextraction Polypyrrole Sol gel Electrochemical deposition Venlafaxine Desorption chamber Ion mobility spectrometry Electrospray ionization

محمدتقي جعفري، محمد سراجي

تقي خياميان، حسن قاضي عسگر