8823

8184

كارشناسي ارشد

بيوتكنولوژي

اصفهان: دانشگاه صنعتي اصفهان، دانشكده كشاورزي

1392

يازده،77ص.: مصور،جدول،نمودار(رنگي)

ص.ع.به فارسي و انگليسي

بدرالدين ابراهيم سيد طباطبايي، مجيد طالبي

16/2/93

سعيد انصاري، مهدي رحيم ملك

مهندسي كشاورزي

ID8184

Micropropagation of Digitalis and Evaluation of Somaclonal Variation in Micro propagated Plants Using SRAP Marker Mahmood Tagheie Palmiramahmood@yahoo com January 1 2014 Department of Agricultural Biotechnology Isfahan University of Technology Isfahan 84156 83111 IranDegree M Sc Language FarsiB E Seyed Tabatabaei Prof Seyedt@cc iut ac irM Talebi Assist Prof mtalebi@cc iut ac irAbstractFoxglove Digitalis sp Scrophulariacae contains cardiac glycoside compounds that digoxin anddigitoxin are still useful drugs for treatment of heart congestive disorders Use of plant cell and tissueculture can be effective in large scale and stable production of these compounds In this study directand indirect regeneration methods in two species of this genus D purpurea and D lanata wereinvestigated In indirect method regeneration through callus regeneration occurred only in D lanatain response of 3mg L 1 BAP and no regeneration observed in callus of D purpurea Use of 13different combinations of plant growth regulators for direct regeneration from node explants showedthat best regeneration in both species occurred in MSmedium supplemented by BAP Number ofshoots produced in direct method in D purpurea was more than D lanata and response of itsexplants to different culture mediums was faster The results showed that phenol production in nodeculture of both species was much less than leaf culture in indirect regeneration and use of phenolabsorbing compound is not necessary Rooting was rapidly achieved even in mediums withoutauxins and 1 mg L 1Zeatin Rooted plantlets transported into the soil and acclimated in greenhouse Regarding to low production of phenol and high number of shoots in direct regeneration it preferredthan indirect regeneration The basic objective of micropropagation is to produce true to type plants therefore it is important to certify and certain the fidelity of produced regenerants Considering highefficiency of node explants regeneration 10 node regenerated plants were selected for geneticstability evaluation Somaclonal variation was studied by 6 pairs of SRAP primers The average ofamplified scorable bands of each primer was in range of 6 E6M2 to 30 E4M5 bands Mostmonomorphic pattern in banding was abserved in E4M5 and most polymorphic was in E6M2 Seemed this average of polymorphism is related to different numbers of subculture and be in disposeof BAP in culture medium of explants These results clearly indicated that use of node explants formicropropagation of Digitalis with respect of high genetic stability is useful Key words Foxglove Regeneration Tissue culture Plant growth regulators Somaclonalvariation SRAP

بدرالدين ابراهيم سيد طباطبايي، مجيد طالبي

سعيد انصاري، مهدي رحيم ملك