پديد آورنده :
نقاش زرگر، الهام
عنوان :
طراحي و مدل سازي داربست نخ هيبريدي ابريشم/ پلي 3- هيدروكسي بوتيرات جهت مهندسي بافت تاندون و ليگامنت
گرايش تحصيلي :
تكنولوژي نساجي
محل تحصيل :
اصفهان: دانشگاه صنعتي اصفهان، دانشكده نساجي
صفحه شمار :
[يازده]، 114ص.: مصور،جدول،نمودار
يادداشت :
ص.ع.به فارسي و انگليسي
استاد راهنما :
داريوش سمناني
استاد مشاور :
سعيد كرباسي
توصيفگر ها :
شبكه هاي عصبي مصنوعي , الگوريتم ژنتيك , اجزاء محدود
تاريخ نمايه سازي :
25/4/93
استاد داور :
حسين حسني، محمدرضا احمدزاده، بتول هاشمي، مسعود لطيفي
كد ايرانداك :
ID644 دكتري
چكيده انگليسي :
۵ Abstract Ruptures and other injuries to tendons and ligaments are among the most common damages to the body specifically in the young and physically active population Recently tissue engineering TE has been recognized as a new approach to regenerate damaged tissues instead of commonly used surgical grafts such as biological and synthetic grafts On the other hands some shortcomings of tendons and ligaments restoration may be overcome through tissue engineering methods The aim of this research is to model and design the silk P3HB hybrid yarn scaffold for tendon and ligament tissue engineering First of all a neural network method used to estimate mechanical properties of multilayer twisted scaffold Then a genetic algorithm model was developed for automation and optimization designing scaffold and afterward a computer model of finite elements method was used for simulation So variable parameters including the number of filament and the number of twist in each layer of 4 layer twisted scaffold were defined and according to Taguchi orthogonal matrix the experiments were designed After that the designed neural network was trained based on the back propagation algorithm The best neural network was formed from one hidden layers in which the hidden layer has 8 3 neurons for output layer 8 neurons for input layer Tangent hyperbolic sigmoid and linear was used as activation functions In following step genetic algorithm method was applied to optimize the main characteristics successfully with 10 error in comparison with real values of tissues Finally a hybrid scaffold made of silk fibroin and P3HB electrospun fibers was designed It was necessary to optimize the electrospinning parameters for P3HB solutions Optimized conditions of electrospinning were found by using statistical analysis The concentration voltage distance spinneret collector and solution flow rate conditions were optimized as 5 10 kv 20 cm and 0 5 ml h for P3HB solution Then the hybrid scaffolds were produced and physico mechanical properties as well as in vitro cell culture were experienced The results of FTIR tests showed that there is no any interaction between silk fibroin and P3HB electrospun fiber in structural formation of hybrid scaffold where both basic polymers remain in the final scaffold independently Also both structures including silk fibroin and hybrid samples made of P3HB and silk were manufactured to evaluate the growth of L929 cells while no toxicity was proved in all samples according to in vitro cytotoxicity The results of cytocompatibility tests show that the samples combined with P3HB electrospun fibers have more cell growth than SF sample in longer time 3rd day and they can be a promised substrate for L929 cells Key words Tendon and ligament tissue engineering Artificial neural network Genetic algorithm Finite element method Hybrid yarnPDF created with pdfFactory Pro trial version www pdffactory com
استاد راهنما :
داريوش سمناني
استاد مشاور :
سعيد كرباسي
استاد داور :
حسين حسني، محمدرضا احمدزاده، بتول هاشمي، مسعود لطيفي
