توليد داربست نانو ليفي عروقي و بهينه سازي برخي از خصوصيات مكانيكي و بيولوژيكي آن

چكيده انگليسي :

Fabrication of Vascular Nanofibrous Scaffold and Optimizing Some of Its Mechanical and Biological Properties Elham Vatankhah e vatankhah@tx iut ac ir Department of Textile Engineering Isfahan University of Technology Isfahan 84156 83111 IranDariush Semnani1 d semnani@cc iut ac irShahnaz Razavi2 razavi@med mui ac irMohammad Morshed1 morshed@cc iut ac irAbstractCoronary artery disease is the most frequent cause of almost half of all deaths in developed countriesand is responsible for 25 of mortality in developing countries Tissue engineering is a promisingtreatment for vascular tissue regeneration The nano fibrous scaffold is considered as the bestbiomimetic environment due to its morphological and dimensional similarity to the fibrillar structureof native ECM The elasticity of biomaterial scaffolds is crucial for their efficacy in tissue engineering In current study we aimed to fabricate electrospun Tecophilic TP gelatin scaffolds for vasculartissue engineering To investigate the effect of scaffold stiffness towards the behavior of SMCs composite scaffolds with different elastic moduli should be produced Determining the most effectiveparameter affecting the elastic modulus of electrospun scaffolds is required to produce a limitednumber of scaffolds with a wide range of elastic modulus values An artificial neural network ANN based model was developed to analyze the nonlinear effects of polymeric composition fiber diameterand fiber alignment on the elastic modulus of electrospun PCL gelatin scaffolds PCL was used insteadof TP in modeling section of this study due to its elastic behavior in body temperature like TP inaddition to its commercial availability at relatively low cost The results of modeling showed the blending ratio of the polymers as the most important factoraffecting elastic modulus of a composite electrospun scaffold Therefore composite scaffolds ofTP gel with weight ratios of 100 0 70 30 50 50 and 30 70 were electrospun which lead to differencesnot only in scaffold stiffness but also in RGD ligand density of TP gel scaffolds Scaffold stiffnessand relative presence of ligand density within the fibers were evaluated using uniaxial tensile test andFTIR analysis respectively Proliferation and morphology of SMCs as well as expression ofcontractile proteins and collagen synthesizing by SMCs were assayed to study the simultaneous effectsof ligand density and stiffness of the substrates on the phenotypic plasticity of SMCs Resultsindicated that although the substrate with both higher ligand density and lower stiffness could enhanceSMC contractility and reduce cell proliferation the stiffness of the scaffold was dominant parameter indirecting the contractile behavior of SMCs Thereafter electrospun TP 70 gel 30 scaffold waschosen as the optimal scaffold for further investigation Porosity surface roughness hydrophilicity and degradability were evaluated for electrospunTP 70 gel 30 scaffolds Blood compatibility of the scaffold was confirmed after hemolysis test andplatelet adhesion assay which is due to hydrophilic structure of the scaffold as well as incorporation ofTP as a blood compatible polymer within the composite fibers A small diameter 5 mm tubularscaffold of TP 70 gel 30 was also fabricated and its mechanical properties were measured whichmanifested its J shaped stress strain behavior similar to that of native arteries with values ofcompliance burst strength and suture retention force comparable to those of native blood vessels Keywords vascular tissue engineering nanofibrous scaffold neural network modeling smoothmuscle cells scaffold stiffness 1 Department of Textile Engineering Isfahan University of Technology Isfahan 84156 83111 Iran2 Department of Anatomical Sciences and Molecular Biology School of Medicine Isfahan University of Medical Sciences Isfahan 81744 176 Iran

وطن خواه، الهام

توليد داربست نانو ليفي عروقي و بهينه سازي برخي از خصوصيات مكانيكي و بيولوژيكي آن

مهندسي بافت عروق خوني , مدل سازي شبكه عصبي , سلول هاي عضلاني صاف , سفتي داربست