تهيه نانو ذرات كراتين با استفاده از روش الكترواسپري و بررسي تجربي و تئوري رهايش داروي دوكسوروبيسين از نانو ذرات حاصل

چكيده انگليسي :

51 Preparation of Keratin nanoparticles by using electrospray method and theoretical and experimental investigation of Doxorubicin release from nanoparticles Saeed Torkian s torkian@ch iut ac ir August 00 2014 Department of Chemistry Isfahan University of Technology Isfahan 84156 83111 Iran Degree M Sc Language FarsiProf T Khayamian Email taghi@cc iut ac ir Abstract In this research the preparation of biodegradable particles of keratin containinganticancer drug Doxorubicin and the evaluation of drug release were studied For thispurpose solutions containing 16 20 and 34 Doxorubicin in Formic acid solvent wereprepared The nanoparticles were generated by using electrospray method Selected rangefor distance of the needle tip to collector was 25cm voltage is 20 kV and feed rate was 0 1ml h Diameter of the nanoparticles containing 20 anticancer drug was 50 nm By usingFourier transform infrared spectroscopy FT IR Formation of possible reaction betweenkeratin and drug also remaining solvent in the nanoparticles was evaluated FT IR resultsshowed that between Doxorubicin drug and keratin no reaction was occurred duringelectrospray and there was no Formic acid remained in the nanoparticle In order toinvestigate the effects of adding Doxorubicin on the microstructure of keratin nanoparticlesused X ray diffraction analysis XRD results showed that Doxorubicin as a crystal placedin the context of crystalline keratin Drug release from nanoparticle containingDoxorubicin was evaluated in phosphate buffer with pH 7 4 UV Vis spectroscopymethod was used to determine the amount of Doxorubicin in phosphate buffer Resultsshows that drug concentration did not influence on drug release mechanism of thenanoparticles Doxorubicin was release from nanoparticle with fick mechanism and withincreasing concentrations of the drug release rate was increased According to the resultswe can say keratin nanoparticles containing Doxorubicin have the ability to control thedrug release and can be used as a delivery system for controlled Doxorubicinchemotherapy Also Doxorubicin interactions with human albumin serum and keratin havebeen studied theoretically by using molecular docking and Ligplot and results showed twotypes of hydrogen and hydrophobic interactions for both Also showed Doxorubicin druginteractions with HSA stronger than keratin that has provided favorable conditions toseparate the drug from the keratin in the blood and binds to HSA Doxorubicin interactionswith FS DNA have been studied theoretically by using molecular docking and Ligplot andPractically was investigated by using UV Vis spectroscopy and the binding constantobtained for doxorubicin and FS DNA from theoretical and practical was consistented Key Words Nanoparticles Doxorubicin Keratin Drug Release

تركيان، سعيد

تهيه نانو ذرات كراتين با استفاده از روش الكترواسپري و بررسي تجربي و تئوري رهايش داروي دوكسوروبيسين از نانو ذرات حاصل