9478

8760

كارشناسي ارشد

مهندسي شيمي

اصفهان: دانشگاه صنعتي اصفهان، دانشكده مهندسي شيمي

1393

يازده،56ص.: مصور،جدول،نمودار

ص.ع.به فارسي و انگليسي

حميد زيلويي، شهره مشايخان

مريم حاج عباس

27/10/93

سعيد نوري خراساني، مرتضي صادقي

مهندسي شيمي

ID8760

Consideration of the Influence of Crosslinker Ratio and Collagen Concentration on Storage Modulus and Pore Size in preparation of collagen membranes Mehdi Aezi Noori mehdi aezi@gmail com September 14th in 2014 Department of Chemical Engineering Isfahan University of Technology Isfahan 84156 83111 IranDegree M Sc Language FarsiSupervisor Dr Hamid Zilouei Email address hzilouei@cc iut ac ir AbstractGenerally guided bone regeneration uses membranes to separate the wound from invading soft tissue cells inorder to provide a proper place for bone cells to proliferate and function Ideally a membrane should bebiodegradable own proper physical characteristics porous and non toxic Biodegradability of the membraneprevents from second surgery in order to be extracted In our current research we chose collagen as our funding material to make a membrane Collagen was dissolvedin 5 0 molar acetic acid for 24 hours freeze dried and then was crossed linked by NHS and EDC solutionsusing MES buffer It was then washed and freeze dried again In this experiment by performing Fouriertransform infrared spectroscopy FTIR for crosslinked and non crosslinked samples it was verified that thebonds in the crosslinked sample was much more strengthened in compare with the non crosslinked one Afterwards by performing mechanical test with Anton Paar rheometer and SEM analysis it was concluded thatamong 5 10 and 20 mg ml concentrations of collagen the latest revealed the best results in terms of physicalproperties and pore size Collagen sample derived from 20 mg ml concentration of collagen solution andcrosslinked with the same mole ratio of crosslinker had the pore size with the range of 27 to 96 microns and itis comparable with commercial samples As the effect of the crosslinker ratio on storage modulus had not beenconsidered in previous projects by applying the regarded tests it was also shown that a same ratio of EDC andNHS provides the highest storage modulus The highest storage modulus was 4 58 Mpa and belonged to thecollagen sample derived from 20 mg ml concentration of collagen solution and crosslinked with the same moleratio of crosslinker which showed 37 increase in compare with the same collagen concentration but withdifferent crosslinker mole ratio EDC NHS 2 1 By comparing the SEM images of non crosslinked sampleas a control sample with other crosslinked samples it was concluded that crosslinking causes a much moreregular morphology in collagen structure Additionally crosslinked samples show a wider range of pore sizesin compare with the non crosslinked sample Key Wordsmembrane collagen crosslink pore size

حميد زيلويي، شهره مشايخان

مريم حاج عباس

سعيد نوري خراساني، مرتضي صادقي