تهيه ي ساختار متخلخل از موسيلاژ دانه ي ريحان و ترسيب نانو ذرات داروي ضد سرطان پكليتاكسل در آن با استفاده از دي اكسيد كربن فوق بحراني

چكيده انگليسي :

152Generation of Porous Structure from Ocimum Basilicum Mucilage and Precipitation of Paclitaxel Nano Particles in the Ocimum Basilicum Mucilage using Supercritical Carbon Dioxide Iman Akbari i akbari@ce iut ac ir Date of Submission September 13 2014 Department of Chemical Engineering Isfahan University of Technology Isfahan 84156 83111 Iran Degree DOCTOR OF PHILOSOPHY Language FarsiSupervisor S M Ghoreishi ghoreshi@cc iut ac irAdvisor N Habibi n habibi@cc iut ac irAbstractPlant derived polymers are widely used in the pharmaceutical industry due to their emollient lack of toxicityand irritating nature and low cost Mucilage is plant derived natural polymer which mainly consists ofpolysaccharides proteins and uranides Plant mucilages are pharmaceutically valuable due to theirnontoxicity low cost emollient and nonirritating nature with wide range of applications In the of first partthis work extracted mucilage from basil seeds BSM was dried using supercritical carbon dioxide SC CO2 gel drying process to form a nanoporous structure for biomedical application SC CO2 assisted processconsists of three steps formation of a BSM hydrogel substitution of water with a suitable solvent drying ofthe gel using SC CO2 The developed polymeric structures were characterized by SEM BET and FTIR Using SC CO2 assisted process the BSM nanostructured networks were produced with pore size diametersabout 40 nm without any agglomeration of the high porosity nanostructure polymers Furthermore specificarea of final products was increased from 69 to 92 m2 g by SC CO2 gel drying The results of FTIR datashowed the presence of hydroxyl and carboxyl groups which are indicative of BSM good bio adhesiveproperty Furthermore the FTIR analyses indicated that the nature of the final product did not change duringthe supercritical drying procedure In the other hand basil seed mucilage was dried using supercritical CO2phase inversion technique to form a nanometric structure It was demonstrated that the product morphologycan be controlled by altering the composition of methanol which function as the co solvent in the non solventstream The most homogeneous product 60 nm mean pore size diameter 78 m2 g BET surface area with noagglomeration was obtained with 2 5 methanol In the other part of this work a promising anticancer drug paclitaxel was precipitated in the basil seeds mucilage BSM using supercritical carbon dioxide SC CO2 The employed SC CO2 process in this research is a combination of gas antisolvent and phase inversiontechniques and consists of two steps 1 casting solution preparation a uniform mixture of BSM water paclitaxel and dimethyl sulfoxide DMSO 2 simultaneous generation and precipitation of nanoparticles inBSM structure using SC CO2 as antisolvent The effect of DMSO water ratio 4 and 6 v v pressure 10 16MPa and CO2 addition rate 1 3 mL min on mean particle size MPS particle size distribution PSD anddrug loading efficiency DLE were studied Particle analyses were performed by scanning electronmicroscopy SEM and Zetasizer High performance liquid chromatography was utilized for studying DLE Nanoparticles of paclitaxel MPS of 117 200 nm depending on process variables with narrow PSD weresuccessfully precipitated in BSM structure with DLE of 56 8 78 2 Experimental results indicated thathigher DMSO water ratio pressure and CO2 addition decreased MPS and DLE Keywords Natural polymer Basil seed mucilage Supercritical fluid Paclitaxel Nano particle Drug loading

اكبري، ايمان

تهيه ي ساختار متخلخل از موسيلاژ دانه ي ريحان و ترسيب نانو ذرات داروي ضد سرطان پكليتاكسل در آن با استفاده از دي اكسيد كربن فوق بحراني

ماتريس پليمري , بازده بارگذاري دارو