ساخت و مشخصه يابي داربست نانوكامپوزيتي ليفي پلي كاپرولاكتون - ديوپسايد

چكيده انگليسي :

77 Synthesis and characterization of nanocomposite fibrous polycaprolactone diopside gelatin scaffold for bone tissue engineering Yasamin Hosseini yasaminhosseyni@ymail com Date of Submission 2016 01 11 Department of Materials Engineering Isfahan University of Technology Isfahan 84156 83111 Iran Degree M Sc Language FarsiSupervisor Rahmatollah Emadi remadi@cc iut ac ir AbstractRepair and regeneration of tissue engineering in different organs including bone defectsis important Bone tissue engineering develops structures that follow from natural boneenvironment to provide biological responses in order to regenerate bone defects According to nanocomposite fibrous structure of bone synthesis of polymer matrixscaffold that filled with ceramic as filler can be effective Electrospinning is an effectivemethod to synthesis of fibrous scaffolds that they are similar to extra cellular matrix Inthis research we prepared diopside nanopowder by sol gel method and XRD pattern ofpowder showed good agreement with the standard card of diopside JCDP 00 019 0239 confirming the synthesize of pure diopside powder without any second phase Accordingto Modified Scherrer Equation the crystallite size of diopside powder was in the range of 32nm TEM image also confirmed that diopside nanopowder had uniform particles withwidth distribution 53 63 26 nm In the following we developed polycaprolactone diopside nanocomposite fibrous scaffold with difference diopside contents In order todistinguish the optimum fibrous scaffold fibers morphology mechanical properties degradation rate and bioactivity were evaluated Due to the low hydrophilicity ofsynthetic polymers the optimum scaffold of polycaprolactoe diopside was modified byusing gelatin In the following the effects of this structure on the scaffold morphology physical properties growth and cell adhesion was evaluated The results showed averagefiber size was reduced from 260 74 7 to 816 81 101 5 depending on the diopsidecontent Furthermore diopside nanoparticle could significantly improve hydrophilicity bioactivity In fibrous polycaprolactone 3 wt diopside scaffold ultimate tensilestrength enhanced from 0 6 0 1 MPa to 4 9 0 1 MPa strain at break enhanced from33 9 7 5 to 101 0 4 0 and Tensile modulus enhanced from 5 3 0 9 MPa to 9 8 0 35 MPa that was selected as Optimized scaffold Due to the agglomeration ofnanoparticles at high diopside content mechanical properties were decreased Surfacemodification of polycaprolactone diopside scaffold by using gelatin after swelling inwater acetone for 20 min leaded to decrease water contact angle and from 109 4 0 5degree to 65 9 1 6 degree and increased cell adhesion So polycaprolactone 3 wt diopside gelatin nanocomposite fibrous scaffold with controllable mechanical andbiological properties can be suitable in order to repair of bone defects Keywords Bone Tissue Engineering Electrospinning Polycaprolactone Diopside nanoparticles Gelatin

حسيني، ياسمين

ساخت و مشخصه يابي داربست نانوكامپوزيتي ليفي پلي كاپرولاكتون - ديوپسايد - ژلاتين جهت كاربرد در مهندسي بافت استخوان

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