11179

10285

كارشناسي ارشد

شيمي معدني

اصفهان: دانشگاه صنعتي اصفهان، دانشكده شيمي

1394

هشت، 68ص.: مصور

كاظم كرمي

شادپور ملك پور

مهدي اميرنصر، حسين چيني فروشان

1395/01/29

شيمي

ID10285

Synthesis and characterization of cyclopalladated complexes containing methylbenzyl amine study of their DNA and BSA interaction with site markers andinvestigating biological and anticancer activities cell lines JURKAT MCF7 andPBMC Mina Rafiee Mina rafiee@ch iut ac ir January 13 2016 Department of Chemistry Isfahan University of Technology Isfahan 2261918 IranDegree M Sc Language FarsiSupervisor Kazem Karami Email address karami@cc iut ac irAbstractIn this thesis bimetallic complex Pd2 C N C6H4CH CH3 NH 2 OAc 2 Has beensynthesized and characterized from the combination of palladium acetate and methylbenzylamine ligand by reflux in toluene as solvent This complex was used as a basis for furtherreactions and then two target complexes were prepared and characterized structurally 5 Pd2 C N C6H4CH CH3 NH2 2 OAc 2 6 Pd2 C N C6H4CH CH3 NH2 2 N3 2 7 Pd C N C6H4CH CH3 NH2 CUR 8 Pd2 C N C6H4CH CH3 NH2 2 N3CS2 Complex 3 has been prepared by the reaction of the preliminary complex with curcumin ligand 1 7bis 4 hydroxyl 3 methoxyphenyl 1 6 hepta diene 3 5 di one which is the effective material ofturmeric and complex 4 was prepared by replacing the previous dimer bridge with sodium azide NaN3 in complex 2 and then with CS2 Both complexes were characterized using FT IR andNMR spectrums and elemental analysis In the second part of the project biological activity of the complexes was evaluated by examiningtheir ability to bind to DNA CT DNA using uv Vis and thermal denaturation and according to theexperiments performed intercalation interaction was suggested In this section the interactionbetween two complexes 3 and 4 with BSA was studied As a result of absorption uv spectrumsof protein in the presence and absence of different concentrations of complexes 3 and 4 it wasobserved that reduction and shifting to higher wavelengths indicates the interaction of thecomplexes with BSA and the alterations in the intensity of the absorption band of BSA revealedthat the interactions between BSA and complexes 3 and 4 causes changes in BSAmicroenvironments around the amino acids While investigating the interaction between complexes 3 and 4 with protein using fluorescent spectroscopy the value of quenching rate constant Kq suggested statistical mechanism and the value of quenching constant of stern velmer Ksv for bothcomplexes indicated the proper binding of BSA with complexes 3 and 4 But complex 3 ismore strongly bound to BSA than complex 4 Competitive studies of sitemarkers of ibuprofenand warfarine were also performed to identify the binding site of the complex to BSA and as a

كاظم كرمي

شادپور ملك پور

مهدي اميرنصر، حسين چيني فروشان