11611

10660

كارشناسي ارشد

اصلاح نباتات

اصفهان: دانشگاه صنعتي اصفهان، دانشكده كشاورزي

1395

چهارده،76ص.: مصور،جدول،نمودار

ص.ع.به فارسي و انگليسي

مهدي رحيم ملك، محمد مهدي مجيدي

آقافخرميرلوحي، مهدي قيصري

محمدرضا سبزعليان، مجيد طالبي

1395/08/03

مهندسي كشاورزي

ID10660

77 Variation in Cumin Accessions Using ISSR and SRAP Molecular Markers and Morphological Characteristics under Moisture Stress and Non stress Conditions Venus Nourbakhsh Habibabadi v nourbakhsh@gmail com 2016 9 6 Department of Plant Breeding Isfahan University of Technology Isfahan 84156 83111 IranDegree MSc Language FarsiSupervisors Mehdi Rahimmalek mrahimmalek@cc iut ac ir Mohammad Mahdi Majidi majidi@cc iut ac irAbstractThis experiment was conducted to investigate the genetic variation in different cumin accessions under normaland water deficient conditions and was carried out according to a randomized complete block design with tworeplicates for each environment at the agriculture experimental station of Isfahan university of technology in2014 2015 The result of analysis of variance for normal condition showed significant genetic variation for seedyield and it s components and all of studied morphological traits between the accessions Drought stress hadsignificant effects on morphological traits The heritability of traits was estimated to be more than 50 andindicated that most of this variation are due to genetic effects Among all yield components umbel per plant andseed per umble had the most correlation with seed yield at both moisture conditions The result of stepwiseregression for normal condition showed that umbel per plant defined most of the variation for yield in thismanner at drought stress seeds per umbel defined most of the variation for yield and can be used as an index forselection Principle component results for both moisture levels showed three components justified more than70 of total variations The resulting clusters divided accessions into three groups and PCA results largelyconfirmed cluster analysis In most cases the accessions were not classified according to their geographicalorigins In molecular study genomic DNA was extracted from fresh leaf samples and PCR reaction performedfollowing measurement of quality and quantity of extracted DNA Out of 10 primer combinations 188 bandswas created of which 162 86 17 were polymorphic Average amount of PIC 0 47 represents suitable abilityof SRAP markers to determine genetic diversity in cumin The genetic distance among groups was obtainedbetween 0 04 and 0 14 regarding within groups variation and variation among accessions results indicated thatthe most similarity was obtained between 4 Joupar Kerman and 3 Marave Tappeh Golestan while the lowestone belonged to 4 Joupar Kerman and 22 Estahban Fars Numerical value of 0 13 obtained for gene flowbetween groups Nm showed that there is gene flow in groups but this current cannot lead to homogenization ofthe groups The mean of shanon information index was 0 44 for primer compositions In the cluster analysis ofSRAP data dendrogram could not classify accessions according to their geographical origins In other word commixture of groups in the dendrogram showed that there is not any genetic diversity among groups Principalcoordinate analysis PCoA of SRAP data showed that four PCs could represent 65 79 of the total variation Keyword Cumin Genetic Variation SRAP markers Accessions

مهدي رحيم ملك، محمد مهدي مجيدي

آقافخرميرلوحي، مهدي قيصري

محمدرضا سبزعليان، مجيد طالبي