12164

11142

كارشناسي ارشد

شيمي تجزيه

اصفهان: دانشگاه صنعتي اصفهان، دانشكده شيمي

1395

ده، 62ص.: مصور، جدول، عكس(رنگي)، نمودار

ص. ع. به فارسي و انگليسي

تقي خياميان، محمدتقي جعفري

كاظم كرمي

علي اصغر انصافي، محمد سراجي

1395/11/26

كتابنامه

شيمي

شيمي

ID11142

ج جذب كمپلكس پاالديم بر روي نانوذرات و محاسبهي بازده آن ۱۴ د بررسي رهايش كمپلكس پاالديم از نانوذرات در محيط آزمايشگاهي ۸۴ ر بررسي برهمكنش بين كمپلكس جذب شده روي نانوذرات BSA و DNA چهارگانه و مقايسهي آن با حالت آزاد كمپلكس ۳۴ ۹ 2 آزمون ۱1 MTT ۹ ۹ جمع بندي و نتيجهگيري ۵۱ ۹ ۴ پيشنهادات و آينده نگري 2۱ منابع و مراجع ۴۱ چكيده انگليسي ۵۶ هشت

61 Interaction between G quadruplex DNA and Pd II complex using spectroscopic methods and release of complex from BSA and Chara algae cellulose NPs and anticancer effect of complex on MCF 7 cell lines Nazanin Hashemzadeh N Hashemzadeh@ch iut ac ir January 2017 Department of chemistry Iran Isfahan University of Technology Isfahan 84156 8311Degree M Sc Language FarsiSupervisors Prof T Khayamian Dr M T Jafari Advisor Dr K Karami Department of chemistry Isfahan University of Technology Isfahan Iran Email Taghi@cc iut ac ir Department of chemistry Isfahan University of Technology Isfahan Iran Email Jafari@cc iut ac ir Department of chemistry Isfahan University of Technology Isfahan Iran Email Karami@cc iut ac ir Abstract The aim of this study is to investigate of complex Pd3 C N C6H4C Cl NO 4 6 in free mode andloading on nanoparticles on stabilizing a portion of telomeric DNA called Gquadruplex DNA with thesequence of 5 G3 T2AG3 3 3 By titration absorption methods the binding constant between complex and G q DNA was found to be Kb 4 5 106 M 1 and showed that this interaction is more selective than ligand Kb 3 5 105 M 1 or duplex DNA Kb 1 5 105 M 1 Type of interaction using titration absorption thermaldenaturation and fluorescence competitive experiments was investigated and all three represented the groovebinding The thermodynamic parameters were measured by thermal denaturation experiment and the negativevalue of G 8 24 kcal mol 1 indicated spontaneously interaction and represented with increasing in theconcentration of the complex from 1 to 120 M melting denaturation was shifted and increased therefore interactions was bimolecular and also confirmed complex as an stabilizer on G q DNA structure In order tomake more effective use of the drug and remove undesirable side effects drug delivery system withnanoparticles was chosen The reason of synthesis BSA nanoparticles and cellulose as drug delivery system isbiodegradable biocompatible properties The complex was loaded on them and then the release of drug fromNPs was investigated in vitro and were compared with each other and with complex free form The resultsshowed BSA NPs were better candidates as carriers because of smaller size and lower release Interaction ofcomplex adsorbed on BSA nanoparticles and G q DNA was also studied and its comparison with the free modeshowed the most effective interaction The number of binding sites was calculated by Job plot technique andwas found to be 2 1 that represented 2 of complexes connected to each G q DNA In the end in biologicalstudies value of IC50 50 M showed that the complex has significant antitumor activities against MCF 7 breast cancer cells then this complex can inhibit telomerase by establish of G q DNA structure Key words G quadruplex DNA Palladium complex BSA nanoparticles Algae cellulose nanoparticles Cytotoxicity

تقي خياميان، محمدتقي جعفري

كاظم كرمي

علي اصغر انصافي، محمد سراجي