پديد آورنده :
ترك زاده، زهرا
عنوان :
استخراج و غني سازي آنزيم سوپراكسيد ديسموتاز از جگر مرغ با بكارگيري افرون كلوئيدي گازي
مقطع تحصيلي :
كارشناسي ارشد
گرايش تحصيلي :
مهندسي شيمي
محل تحصيل :
اصفهان: دانشگاه صنعتي اصفهان، دانشكده مهندسي شيمي
صفحه شمار :
سيزده، 86ص.: مصور، جدول، نمودار
يادداشت :
ص. ع. به فارسي و انگليسي
استاد راهنما :
كيقباد شمس
توصيفگر ها :
افرون كلوئيدي گازي , پايداري , غني سازي آنزيم سوپراكسيد ديسموتاز , روش شناسي رويه پاسخ
استاد داور :
محمد اميري، مهدي كديور
تاريخ ورود اطلاعات :
1395/12/15
رشته تحصيلي :
مهندسي شيمي
چكيده انگليسي :
Extraction and enrichment of Superoxide dismutase from chicken liver using colloidal gas aphrons Zahra Torkzade z torkzadeh@ce iut ac ir Department of chemical engineering Isfahan University of Technology Isfahan 84156 83111 IranDegree M Sc Language FarsiSupervisor K Shams Professor k shams@cc iut ac irAbstractColloidal gas aphrons are stable micro bubbles with a multi layered shell that is generated by severely stirring anaqueous surfactant solution The high interfacial area introduced by the small size of aphron bubbles and highstability of colloidal gas aphrons due to the presence of a thin soapy shell surrounding the core of the bubbles make them interesting in various processes such as protein recovery The purpose of this research is enrichmentof superoxide dismutase from affordable and available chicken liver Aphron was generated by severely stirringpolysorbate 20 solution using a homogenizer One of the most important properties by which the quality ofaphrons is assessed is stability Surfactant concentration homogenizer speed stirring time and fraction of thedistance of homogenizer s disperser from the surface of the solution are chosen as operational variables affectingthe quality of aphron generation Optimization is performed based on the stability of aphron For experimentaldesign data modeling and optimization of the operational parameters minitab17 software and response surfacemethodology is used Optimal values of surfactant concentration homogenizer speed stirring time and fractionof distance of the homogenizer s disperser from surface of the solution are determined as 7 5 mM 24000 rpm 9min and a quarter of total solution s height from surface of the solution respectively Under optimal operationalconditions aphron was generated and used for protein recovery Surfactant concentration pH and volume ratioof aphron to enzyme solution were chosen as operational variables affecting the enrichment ratio Optimal valuesof surfactant concentration pH and volume ratio of aphron to enzyme solution were obtained as 10mM 4 and1 8 respectively Activity of SOD was measured Electrophoresis results indicated that the activity of the enzymeis preserved Key words Colloidal Gas Aphron Stability Superoxide dismutase enrichment ResponseSurface Methodology
استاد راهنما :
كيقباد شمس
استاد داور :
محمد اميري، مهدي كديور
