15361

1520 دكتري

دكتري

بيومواد

اصفهان : دانشگاه صنعتي اصفهان

1398

و. 93 مصور، جدول، نمودار

مهدي احمديان، محمد رفيعي نيا

رحمت الله عمادي ، شيدا لباف، سعيد كرباسي

1398/09/26

كتابنامه

مواد

مهندسي مواد

1398/09/26

2586479

AbstractBone morphogenetic protein 2 has a major role in promoting bone regeneration intissue engineering scaffolds Growth factor release rate is a remaining crucialproblem in these systems The aim of this study was to fabricate and characterize a novel calciumphosphate polyphosphazenes porous scaffold for the sustained release of bonemorphogenetic protein 2 in bone tissue engineering Polyphosphazenes weresubstituted with 2 dimethylaminoethanol and evaluated by GPC NMR and in vitrodegradation Calcium phosphate porous samples were prepared fromhydroxyapatite nanoparticles and naphthalene using the sintering method at 1250 C before being composited with poly dimethylaminoethanol phosphazenescontaining chitosan microspheres loaded with bone morphogenetic protein 2 Thecharacteristics and biodegradability of the product were evaluated by SEM XRD and in vitro degradation Moreover the release rate and mechanical properties ofthe scaffolds were investigated The release behavior was found to be sustained since the scaffolds had beenfabricated from polyphosphazenes with a low degradation rate The release rates ofthe scaffolds were observed to increase with increasing chitosan microspherescontent from 10 to 30 The bioactivity of the scaffolds depended on the releaserate of growth factor while bone morphogenetic protein 2 was able to induce anosteoblast proliferation The results of cell adhesion and cell viability tests showedthat scaffolds displayed a non toxic behavior and western blot analyses confirmedthat the scaffolds loaded with growth factor increased the osteogenic differentiationpotential of cells when compared with scaffolds alone These results demonstratethat these scaffolds can be successfully utilized in bone tissue engineering Keywords Bone tissue engineering Controlled biodegradation Sustained release Polyphosphazenes Bone morphogenetic proteins

مهدي احمديان، محمد رفيعي نيا

رحمت الله عمادي ، شيدا لباف، سعيد كرباسي