Epidermolysis bullosa (EB) is a type of genetic disorder that causes blisters and ulcers on the skin. The purpose of this study is to make and characterize a double-layered wound dressing based on tetraethylarthosilicate prepared by Bahayar Sanat as a support layer and a layer of nanolipizine and sodium alginate loaded with propolis. Nanofibrous layers with concentrations of 0, 20,30 and 40% by weight of propolis were prepared and eva‎luated. According to the results, the increase in propolis concentration increases the uniformity of the morphology of nanofibers and 106.nm 1686 and the smallest diameter was 1686 nm, the maximum diameter obtained was 3.6 N/mm2, the tensile strength of the layers has improved due to the increase in propolis. Tensile strength was confirmed in the highest state of preservation of the nature of the material. FTIR came. The increase of propolis had no specific effect on the crystallinity percentage of the samples. Based on the test, they were recognized as biocompatible in 24 and 48 hours, and the presence of propolis caused the MTT of the substrates prepared based on the test. The addition of propolis and the presence of sodium alginate caused Staphylococcus aureus to develop antibacterial properties against the hydrophilic bacteria, and the contact angle for the obtained nanofibers reached 20 degrees. The surface roughness of the prepared layers changes in the range of 350 to 560 nm.

افسانه ولي پوري، عبدالكريم حسيني

منيره كوهي

فرزانه علي حسيني، داريوش سمناني