Production of all female stock populations can have many benefits in the rainbow trout (Oncorhynchus mykiss) farming industry. To produce it, undifferentiated larvae are exposed to androgens, which results in fish with XX (female) testis genotypes. The sperm obtained from these fishes are fertilized with normal eggs to all females population be created. The aim of this study is to separate the sex-changed male rainbow trout from the normal rainbow trout in the population of both sexes by examining the SdY gene and examining the quality of sperm in these two treatments. The septation of opened-duct sex-changed rainbow trout from normal rainbow trout was done using SdY gene specific primers and electrophoresis. The investigated indicators include percentage of motile sperm (MOT), motility time, sperm density and biochemical indices of sperm plasma (glucose, fructose, calcium, protein, magnesium, chlorine, potassium) during the reproduction season in rainbow trout was also checked. The results of this study show that the use of specific primers for the SdY gene is effective and also the investigated sperm parameters had no significant difference except for the sperm volume which was measured as 8.8 ml in transsexual rainbow trout and 18.3 ml in normal rainbow trout. (P<0.05).

سالار درافشان , فاطمه پيكان حيرتي

اميرحسين جلالي، يزدان كيواني