Bone tissue engineering is a novel method for repairing and regenerating bone tissue in order to overcome the challenges of treating severe bone injuries. In this regard, the use of scaffolds with suitable physical, chemical, mechanical and biological properties can create a constructive effect in the process of reconstruction. In the current research, the aim is to create a three-dimensional hydrogel structure based on sodium-alginate-gelatin by freeze-drying method. In order to accelerate the ossification process, bioactive glass nanoparticles were prepared by sol-gel method and used in hydrogel structure. The effect of these particles on all properties of the produced hydrogel structure was eva‎luated. In order to investigate the chemical, mechanical and physiological properties, sodium-alginate-gelatin biocomposite hydrogel scaffolds containing 0, 5 and 10% by weight of bioactive glass nanoparticles were synthesized. By adding nanoparticles, the mechanical properties of hydrogels increased compared to the group without glass. The compressive modulus and toughness of the samples in the glass-free group increased from 7.3 KPa and 6.01 MPa to 8.9 KPa and 7.74 MPa in the 5% group. The results of the wettability test showed that the addition of nanoparticles decreases the wetting angle, so that these angles in the group without glass are 73 degrees, and with the addition of 5% and 10% by weight, this angle becomes 53 and 46 degrees, respectively. Also, the results of scanning electron microscope images and BET analysis showed that the addition of glass nanoparticles reduces the size and diameter of the pores in the scaffolds. The results obtained from the swelling and degradability test of the scaffolds showed that the addition of nanoparticles improves the swelling in the chemical composition and reduces the degradability, so that the amount of swelling in the 5% and 10% groups increased by 647% and 183%, respectively. Compared with the group without glasses, it showed. In addition, the percentage of degradation in the two groups containing glass is controllable and predictable compared to the group without glass. The bioactivity results of the scaffolds during 28 days of immersion in the simulated body solution show the formation of layer deposits. Apatite in two groups contained bioglass. The biocompatibility of the scaffolds using the Alamarblue test over a period of 5 days showed that all groups were biocompatible and the 5% bioglass group was the optimal sample for use in bone tissue.

شيدا لباف , فتح اله كريم زاده

محمدحسين عنايتي , مهران نحوي