Cancer is known as the second leading cause of death after cardiovascular diseases, and cervical cancer is the second most common disease among women worldwide. The most common method of cancer treatment is chemotherapy in which drugs such as cisplatin are used to destroy cancer cells. The disadvantages of chemotherapy methods have led to the development of local and systematic methods. The aim of this research is to fabricate and characterize chitosan-dopamine nanocomposite hydrogel adhesive containing different concentrations of bafolic acid modified mesoporous silica nanoparticles (1, 2, 5 and 7% NPs) loaded with cisplatin drug for the treatment of cervical cancer. In this regard, first mesoporous silica nanoparticles with spherical morphology and mesoporous cavities were modified for drug loading using Stober synthesis method and then using folic acid. Also, chitosan was separately modified with dopamine groups. After characterizing the particles and the chitosan/dopamine precursor, mesoporous silica nanoparticles were added to the hydrogel substrate with the mentioned concentrations and characterized. The results showed that the modification of mesoporous silica nanoparticles with folic acid led to the creation of new functional groups and a change in the size of the nanoparticles from 116 ± 20 nm to 126 ± 25 nm. Also, the measured surface charge changed from -42 mV to -32 mV in the modification of particles with folic acid. Next, in order to eva‎luate the effect of mesoporous silica nanoparticles in hydrogel adhesive, the mechanical properties, adhesive properties, drug release and biocompatibility of hydrogels were investigated. In this regard, increasing the concentration of particles up to 2%, increasing the mechanical properties compared to pure chitosan/dopamine hydrogel adhesive. showed Further, increasing the concentration of particles up to 2% led to a decrease in the measured adhesion strength compared to pure chitosan/dopamine hydrogel adhesive using cow scalp, and further increasing the concentration of particles up to 7% increased the adhesion strength to chitosan hydrogel adhesive. /dopamine-mesoporous silica nanoparticles. Cell eva‎luation was also performed on two types of healthy L929 cells and Hela cancer cells. At a concentration of 5%, Hela cancer cell toxicity was shown to be 88.7±10.5% in the first 24 hours and 85.6±4.6% in 72 hours after cultivation, due to the accumulation of cisplatin in Hela cancer cells. Also, after 3 days at a concentration of 5%, cell viability in L929 fibroblast cells decreased to 94.9±1.08% due to the adverse effect of nanoparticles in higher concentrations and long exposure time. Next, the clumping of nanoparticles at a concentration of 5% leads to less cumulative release of cisplatin, and at concentrations of 1 and 2%, the presence of more space between the particles facilitated the release of the drug from the particles and caused more drug release.

مهشيد خرازيهاي اصفهاني , حميدرضا سليمي جزي

زهرا طالبي مزرعه شاهي , محمد خدائي