Skin cancer remains an urgent global health concern that requires innovative approaches for an effective treatment. In this study, a green synthesis method was applied for the synthesis of titanium dioxide (TiO2) nanoparticles using aloe vera plant extract. The synthesized nanoparticles were characterized using a range of techniques including scanning electron microscopy (SEM), transmission electron microscopy (TEM), UV-Vis spectroscopy, Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), dynamic light scattering with Zeta potential analysis (DLS-Zeta) and energy dispersive X-ray spectroscopy (EDX). To create an optimized structure based on particle size and size distribution, various parameters were investigated. According to the results of TEM and SEM images, the size of the titanium dioxide nanoparticles was found to be in the range of 20-60 nm. According to UV-Vis spectroscopic analysis, the produced nanoparticles demonstrated light sensitivity in the UV, visible, and even near-infrared light ranges. X-ray diffraction (XRD) patterns of the synthesized nanoparticles showed the crystal structure of anatase phase. Upon optimization of nanoparticles, nanofibers were prepared using a blend of polycaprolactone (PCL) and chitosan polymers through electrospinning technique. To increase the therapeutic potential of the scaffolds, TiO2 nanoparticles of 2, 5, 10, 15 wt% were incorporated within the nanofiberous structure. The morphological characteristics of the composite scaffolds were eva‎luated using SEM and FTIR. The SEM images of the scaffolds revealed a diameter below 100 nm with a suitable distribution of fiber diameter. Mechanical testing, including tensile strength eva‎luations, was performed to measure the effect of nanoparticle incorporation on scaffold integrity. Biodegradability, drug release, MTT assay, photothermal, antioxidant and antibacterial characteristics were also studied. The sum of results presented in this study, demonstrate the successful synthesis of TiO2 nanoparticles through a green approach and their effective incorporation into PCL-Chitosan scaffolds. Furthermore, through the addition of curcumin drug (Cur) to the optimal scaffold, a suitable combined treatment method (photothermal + drug release) for the skin cancer was obtained. This multifaceted approach offers a promising way to develop advanced materials with potential applications in skin cancer therapy.

شيدا لباف , مريم كرباسي

مهشيد خرازيهاي اصفهاني , افسانه فخار