Enhancing bone defect repair using Ti6Al4V scaffolds produced by additive manufacturing presents a promising approach to significantly improve bone regeneration and accelerate healing. In this study, bulk Ti6Al4V samples fabricated via selective Laser Melting (SLM) and Electron Beam Melting (EBM) were initially examined. The objective of this work is to compare these samples based on their microstructural, mechanical, and electrochemical anisotropic properties (in the -x, -y, and -z planes) for Ti6Al4V SLM-X2, SLM-XY, and EBM samples. Experimental results indicated that SLM materials exhibit higher corrosion resistance than EBM. Specifically, the (-x) plane in SLM samples showed the highest corrosion resistance (71.202 Ω·cm²) among all samples tested. Subsequently, Ti6Al4V scaffolds produced via EBM were modified in an alkaline solution to develop bioactive anatase and rutile phases. Wettability testing showed that the modified surface exhibited a contact angle of less than 11.5°, demonstrating enhanced hydrophilicity. Next, a composite biopolymer carrier (gelatin/alginate) containing insulin-like growth factor-1 (IGF-1) was applied to the samples via the dipping method. Results demonstrated that the modified porous Ti6Al4V scaffolds significantly increased cell viability (from 80.7% to 104.1%) and cell proliferation, indicating strong potential for bone defect applications. This study suggests that porous scaffolds coated with Gel/Alg-IGF-1 could be a valuable option for orthopedic implants. In the final phase, to prevent infections and further enhance bone regeneration in the porous Ti6Al4V scaffolds, layer-by-layer self-assembly systems were applied with single and multilayer carriers containing a combination of Gel/Alg-IGF-1 and Chi-Cef on the surface of the porous implants. The release profiles of IGF-1 and cefazolin from these carriers were analyzed. Cellular morphology was characterized, and the antimicrobial properties of the materials were eva‎luated. Results revealed that multilayer carriers supported a higher cell count compared to single-layer carriers, while the chemical ratio of gelatin to alginate [(4Gel/Alg-IGF-1) Chi-Cef] in the single-layer carrier closely resembled that of the multilayer samples. Antimicrobial assessment demonstrated that increasing the number of polymeric carrier layers in the layer-by-layer system produced an inhibition zone with a maximum diameter of 16.6 ± 0.6 mm. This study eva‎luated drug-release control systems designed to enhance MG63 cell behavior and prevent bacterial accumulation in orthopedic applications.

مسعود عطاپور

محمد خدائي , محمدعلي شيخ السلام , عبدالله صبوري

فاطمه داور , عليرضا علافچيان , حسين صالحي