Oral diseases, including recurrent aphthous stomatitis, are chronic inflammatory conditions that are often characterized by erosive or painful oral lesions and have a significant impact on the quality of life. In recent years, the use of bioadhesives based on various adhesive polymers have attracted a lot of attention. In this regard, the aim of this research is to make a mucous adhesive hydrogel with high adhesive properties and long-term drug release for the treatment of oral diseases. For this purpose, a nanocomposite hydrogel mucosal adhesive based on methacrylated Hydroxypropyl methyl cellulose hydrogel and methacrylated lignosulfonate containing nanostructured lipid carriers was eva‎luated. For this reason, after modifying hydroxypropyl methyl cellulose and lignosulfonate polymers with methacrylate group, hydrogel based on these two with methacrylate lignosulfonate concentrations (0, 2, 6, 10 and 14 wt%) was made and then based on physical properties and Mechanically optimized. Next, nanostructured lipid carriers with and without the presence of alpha-pinene drug were prepared and characterized by solvent diffusion method. After that, nanocomposite hydrogel containing different concentrations of nanostructured lipid carriers (0, 18, 38, 50 wt%) was made and optimized. From various tests such as scanning electron microscope (SEM), transmission electron microscope (TEM), Fourier transform infrared spectroscopy (FTIR), nuclear magnetic resonance spectroscopy (NMR), swelling and degradation rate test, compressive and rheological mechanical test, as well as adhesion test. Cow buccal mucosa and antioxidant test as well as drug release and biocompatibility were used to investigate the properties of hydrogels. The results showed that methacrylated Hydroxypropyl methyl cellulose / methacrylated lignosulfonate hydrogels were created by creating covalent bonds through carbonyl groups and chemical binders as well as hydrogen bonds in situ. The addition of nanostructured lipid carriers by creating hydrogen interactions with the polymer chain led to significant control of the physical and mechanical properties of the hydrogel. Also, with the increase in the concentration of lignosulfonate, the adhesion strength of the hydrogel increased and reached 71 kPa in the sample with 10 wt% of lignosulfonate. In this way, the sample containing 10% methacrylic lignosulfonate was selected as the optimal sample and was used in the manufacture of nanocomposite hydrogel. The results showed that the presence of nanostructured lipid carriers up to 18 wt% increased the stability and decreased the degradation rate of hydrogels and increased the compressive strength (1.1 times). In addition, the addition of nanostructured lipid carriers improved the rheological properties, tissue adhesion strength (2 times) and antioxidant properties. The results of drug release for composite hydrogels containing 18 wt% of nanostructured lipid carriers at 37°C showed controlled drug release within 96 hours. The results confirmed that the nanocomposite hydrogel had no toxicity against fibroblast cells and the cells adhered to the surface of the hydrogel, grew and expanded; Therefore, it can be concluded that nanocomposite hydrogel containing 10 wt% of methacrylate lignosulfonate and 18 wt% of nanostructured lipid carriers with mechanical and physical properties suitable for buccal mucosa, antioxidant activities and adhesion, the potential to be used in the treatment of pests It has a mouth.

مهشيد خرازيهاي اصفهاني , فتح اله كريم زاده

الهام خادم

زهرا طالبي مزرعه شاهي , افسانه فخار