ساخت و مشخصه‌يابي داربست‌هاي نانوكامپوزيتي پلي‌كاپرولاكتون/ديوپسيد-آهن/ گرافن كوانتوم دات-كلر به‌منظوركاربرد همزمان مهندسي بافت استخوان و گرمادرماني

Bone defects resulting from surgical tumor resection present a dual clinical challenge: the risk of residual microscopic cancer cells an‎d disruption of natural bone regeneration. To address this, multifunctional nanocomposite scaffolds based on polycaprolactone/iron-doped diopside/chlorine-doped graphene quantum dots (PCL/Fe-DIO/Cl-GQD) were designed an‎d fabricated for simultaneous bone tissue engineering an‎d photothermal cancer therapy. Diopside an‎d iron-doped diopside nanopowders (0.05, 0.15, an‎d 0.25 mol% Fe) were synthesized via the sol-gel method. XRD analysis confirmed pure diopside phase formation, with crystallite size decreasing from 24.1 nm in pure DIO to 18.4 nm at the highest Fe content, indicating controlled lattice substitution. The 0.15Fe-DIO composition showed the best overall performance, with a saturation magnetization of 1.92 emu/g an‎d a specific absorption rate (SAR) of 55.8 W/g, making it the primary can‎didate for magnetic hyperthermia. Cell viability remained at 82–85% after 96 hours, confirming acceptable cytocompatibility. Three-dimensional scaffolds containing 20, 40, an‎d 60 wt% ceramic powder in a PCL matrix were fabricated by robocasting. The P-40%(0.15Fe-DIO) composition was selec‎ted as optimal, exhibiting a compressive strength of 90 MPa — over 200% higher than pure PCL — a Youngʹs modulus of ~105 MPa, interconnected porosity of 70%, an‎d pore sizes of 580–680 µm, well within the range required for cell infiltration an‎d vascularization. Cl-GQDs (mean size: 7.53 ± 2.77 nm) were subsequently synthesized an‎d immobilized onto aminolysis/hydrolysis-functionalized scaffold surfaces, reducing the water contact angle to 38 ± 1° an‎d shifting degradation from bulk erosion to controlled surface degradation. Bioactivity testing in simulated body fluid confirmed strong apatite-forming ability in the final scaffold, with controlled Si an‎d Fe ion release maintained at 8.3 ± 0.3 an‎d 0.38 ± 0.05 mg/L, respectively. Under 808 nm NIR laser irradiation, scaffold temperature exceeded 52°C within 300 seconds. Photothermal treatment reduced MG-63 osteosarcoma cell viability to below 42/8%, compared to 71.3% without irradiation, with confocal microscopy confirming predominant cell death in the irradiated group. The P-40%(0.15Fe-DIO)/AM/HD/Cl-GQD scaffold — combining mechanical integrity, strong bioactivity, controlled biodegradation, an‎d effective photothermal response — is proposed as a multifunctional platform for bone defect regeneration an‎d localized elimination of residual tumor cells following resection surgery.

مهدي احمديان , رحمت اله عمادي

زهرا سبحاني , فريبرز توانگريان

سعيد كرباسي , عليرضا علافچيان , محمد خدايي