3308

3173

كارشناسي ارشد

﴿بيوتكنولوژي كشاورزي﴾

اصفهان: دانشگاه صنعتي اصفهان، دانشكده كشاورزي

1384

دوازده، 106، [II] ص.: مصور، جدول، نمودار

ص. ع. به فارسي و انگليسي

مسعود بهار، خورشيد رزمجو

محمدحسين اهتمام

بدرالدين سيد طباطبايي، فرشيد نور بخش

1396/02/30

كتابنامه

كشاورزي

مهندسي كشاورزي

ID3173

به فارسي و انگليسي: قابل رويت در نسخه ديجيتال

Abstract In order to study genetic structure of strains of Rhizobium leguminosarum bv trifoliirecovered from some species of clover in soils of different parts of Iran soil sampleswere collected from various geographical regions of the country including Lorestan Isfahan Khorasan Hamedan Kermanshah Kordestan and West Azerbaijan Cloverspecies including Bersim clover Persian clover and Red clover were grown in thesesoils Following nodulating soil Rhizobia on the host roots bacteria were isolated fromnodules and a bacterial collection containing 420 isolates was provided Strains of R leguminosarum bv trifolii were characterized using BOX PCR fingerprinting method At first the isolates from each 21 soil species treatments were compared Second stepof comparison was performed among selected isolates of the first step in each soilsample and final step of caparison was done between isolates from different soilsamples The number and frequency of isolates was determined using strain richnessand strain dominance indices Natans soil Persian clover treatment with strain richnessof 0 62 showed the most diversity among all the investigated treatments Kermanshahsoil sample with SR of 0 33 and Lorestan soil sample with the SR of 0 12 were the mostand the least diverse soil samples respectively The values of strain richness for theclover species were estimated 0 3 for Red clover 0 28 for Persian clover and 0 23 forBersim clover From 152 isolates that were investigated in this study 23 strain typesrelating to three clover species recognized which strain number 9 with SR of 0 16 wasthe most dominant strain of all Profile generated by BOX PCR was used to estimategenetic similarity of the strains using Jacard similarity coefficient and a cluster wasconstructed according to UPGMA method at NTSYS 2 0 software Comparison ofsimilarity coefficients and relating dendrogram did not reflect any special relationbetween strain of rhizobia strain clover species and soil sample In order to investigatephylogenetic relationships strains were characterized by RFLP of recA gene Resultsindicated that all the isolates belong to the same specie Also the strains were subjectedto melanin production and salt resistant tests which the results of the two were negative A primer mocDEF mocDER was employed in order to detection of rhizopine activityin the R leguminosearum bv trifolii strains recovered from Iranian clovers None ofthe isolates investigated in this study amplified the desired segment and therefore didnot have the ability of catabolism of this component

مسعود بهار، خورشيد رزمجو

محمدحسين اهتمام

بدرالدين سيد طباطبايي، فرشيد نور بخش