پديد آورنده :
سعيدي، كبري
عنوان :
جداسازي، بيان فراوان و بررسي خصوصيات فيزيولوژيك پروتين شبه تئوماتين از گياه يونجه يكساله
مقطع تحصيلي :
كارشناسي ارشد
گرايش تحصيلي :
بيوتكنولوژي
محل تحصيل :
اصفهان: دانشگاه صنعتي اصفهان، دانشكده كشاورزي
صفحه شمار :
پانزده، 121، [II]ص.: مصور، جدول
يادداشت :
ص. ع. به فارسي و انگليسي
استاد مشاور :
بهرام شريف نبي
توصيفگر ها :
پروتين هاي نوتركيب , قطعات ORF , DNA ژنومي , محصول PCR
تاريخ نمايه سازي :
18/4/86
استاد داور :
بدرالدين ابراهيم طباطبائي، نفيسه نيلي
تاريخ ورود اطلاعات :
1396/02/24
چكيده فارسي :
به فارسي و انگليسي: قابل رويت در نسخه ديجيتال
چكيده انگليسي :
AbstractPathogenesis related proteins PR Proteins are induced in response to environmentalstresses such as osmotic and drought stress wounding microbial infections andtreatment with specific plant hormones and elicitors These proteins are presentlyclassified into several groups PR 1 through PR 17 based on their amino acid sequenceand biochemical functions The present study has been focused on isolation over expression and analysis of the physiological activities of the thaumatin like proteins i e PR 5 in the model plant Medicago truncatula Analysis of the M truncatula genome available freely on the internet indicated the presence of at least six PR 5 Open ReadingFrames ORFs three of which along with two previously reported cDNAs were selectedfor this study Highest homologies for the five ORFs gens dubbed tlp 1 2 3 4 and 5 to known PR 5 proteins are respectively 87 to Arabidopsis thaliana aad02499 63 again to A thaliana aad02499 78 to A thaliana NP 173261 68 to Lycopersicumsculentum L76632 and 56 to either Vitis vinifera Y10992 or Cicer arietinumAJ010501 Using specific primers the sequences were isolated from the M truncatulagenome and all but one were confirmed to match with those previously reported Nosequence was obtained for tlp 4 at time this reported All the fragments were then transformed into Escherichia coli isolate BL21 DE3 using pET21c plasmids forsubsequent overexpression All five genes were expressed as inclusion bodies IBs withmasses estimated by SDS PAGE corresponding to the theoretical values As expectednone of the protein IBs had any detectable effect on phytopathogenic fungi Rhizoctoniasolani Alternaria alternata and Fusarium graminearum However when IBs wererefolded using 4 refolding methods all showed comparable strong antifungal activityagainst these fungi No report on overexpression or analysis of PR 5 family of proteins inM truncatula had been published prior to this project
استاد مشاور :
بهرام شريف نبي
استاد داور :
بدرالدين ابراهيم طباطبائي، نفيسه نيلي
